2017年4月
Hydroxyethyl cellulose matrix applied to serial crystallography
SCIENTIFIC REPORTS
- 巻
- 7
- 号
- 開始ページ
- 45604
- 終了ページ
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1038/s41598-017-00761-0
- 出版者・発行元
- NATURE PUBLISHING GROUP
Serial femtosecond crystallography (SFX) allows structures of proteins to be determined at room temperature with minimal radiation damage. A highly viscous matrix acts as a crystal carrier for serial sample loading at a low flow rate that enables the determination of the structure, while requiring consumption of less than 1 mg of the sample. However, a reliable and versatile carrier matrix for a wide variety of protein samples is still elusive. Here we introduce a hydroxyethyl cellulose-matrix carrier, to determine the structure of three proteins. The de novo structure determination of proteinase K from single-wavelength anomalous diffraction (SAD) by utilizing the anomalous signal of the praseodymium atom was demonstrated using 3,000 diffraction images.
- リンク情報
- ID情報
-
- DOI : 10.1038/s41598-017-00761-0
- ISSN : 2045-2322
- Web of Science ID : WOS:000398545900022