2015年6月1日
Structure determination of an integral membrane protein at room temperature from crystals in situ
Acta Crystallographica Section D: Biological Crystallography
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- 巻
- 71
- 号
- 開始ページ
- 1228
- 終了ページ
- 1237
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1107/S139900471500423X
- 出版者・発行元
- International Union of Crystallography
The structure determination of an integral membrane protein using synchrotron X-ray diffraction data collected at room temperature directly in vapour-diffusion crystallization plates (in situ) is demonstrated. Exposing the crystals in situ eliminates manual sample handling and, since it is performed at room temperature, removes the complication of cryoprotection and potential structural anomalies induced by sample cryocooling. Essential to the method is the ability to limit radiation damage by recording a small amount of data per sample from many samples and subsequently assembling the resulting data sets using specialized software. The validity of this procedure is established by the structure determination of Haemophilus influenza TehA at 2.3 Å resolution. The method presented offers an effective protocol for the fast and efficient determination of membrane-protein structures at room temperature using third-generation synchrotron beamlines.
- リンク情報
- ID情報
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- DOI : 10.1107/S139900471500423X
- ISSN : 1399-0047
- ISSN : 0907-4449
- PubMed ID : 26057664
- SCOPUS ID : 84931025168