2022年5月25日
Lipidic cubic phase serial femtosecond crystallography structure of a photosynthetic reaction centre
Acta Crystallographica Section D Structural Biology
- 巻
- 78
- 号
- 6
- 開始ページ
- 698
- 終了ページ
- 708
- 記述言語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1107/s2059798322004144
- 出版者・発行元
- International Union of Crystallography (IUCr)
Serial crystallography is a rapidly growing method that can yield structural insights from microcrystals that were previously considered to be too small to be useful in conventional X-ray crystallography. Here, conditions for growing microcrystals of the photosynthetic reaction centre of Blastochloris viridis within a lipidic cubic phase (LCP) crystallization matrix that employ a seeding protocol utilizing detergent-grown crystals with a different crystal packing are described. LCP microcrystals diffracted to 2.25 Å resolution when exposed to XFEL radiation, which is an improvement of 0.15 Å over previous microcrystal forms. Ubiquinone was incorporated into the LCP crystallization media and the resulting electron density within the mobile QB pocket is comparable to that of other cofactors within the structure. As such, LCP microcrystallization conditions will facilitate time-resolved diffraction studies of electron-transfer reactions to the mobile quinone, potentially allowing the observation of structural changes associated with the two electron-transfer reactions leading to complete reduction of the ubiquinone ligand.
- リンク情報
- ID情報
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- DOI : 10.1107/s2059798322004144
- ISSN : 2059-7983