論文

査読有り 筆頭著者 国際誌
2013年6月28日

Receptor for activated C-kinase (RACK1) homolog Cpc2 facilitates the general amino acid control response through Gcn2 kinase in fission yeast.

The Journal of biological chemistry
  • Yusuke Tarumoto
  • ,
  • Junko Kanoh
  • ,
  • Fuyuki Ishikawa

288
26
開始ページ
19260
終了ページ
8
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1074/jbc.M112.445270
出版者・発行元
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC

General amino acid control (GAAC) is crucial for sensing and adaptation to nutrient availability. Amino acid starvation activates protein kinase Gcn2, which plays a central role in the GAAC response by phosphorylating the α-subunit of eukaryotic initiation factor 2 (eIF2α), leading to the translational switch to stimulate selective expression of stress-responsive genes. We report here that in fission yeast Schizosaccharomyces pombe, Cpc2, a homolog of mammalian receptor for activated C-kinase (RACK1), is important for the GAAC response. Deletion of S. pombe cpc2 impairs the amino acid starvation-induced phosphorylation of eIF2α and the expression of amino acid biosynthesis genes, thereby rendering cells severely sensitive to amino acid limitation. Unlike the Saccharomyces cerevisiae Cpc2 ortholog, which normally suppresses the GAAC response, our findings suggest that S. pombe Cpc2 promotes the GAAC response. We also found that S. pombe Cpc2 is required for starvation-induced Gcn2 autophosphorylation, which is essential for Gcn2 function. These results indicate that S. pombe Cpc2 facilitates the GAAC response through the regulation of Gcn2 activation and provide a novel insight for the regulatory function of RACK1 on Gcn2-mediated GAAC response.

リンク情報
DOI
https://doi.org/10.1074/jbc.M112.445270
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/23671279
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3696696
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000321335800058&DestApp=WOS_CPL
ID情報
  • DOI : 10.1074/jbc.M112.445270
  • ISSN : 0021-9258
  • eISSN : 1083-351X
  • PubMed ID : 23671279
  • PubMed Central 記事ID : PMC3696696
  • Web of Science ID : WOS:000321335800058

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