2011年
A novel serum-free monolayer culture for orderly hematopoietic differentiation of human pluripotent cells via mesodermal progenitors.
PloS one
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- 巻
- 6
- 号
- 7
- 開始ページ
- e22261
- 終了ページ
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1371/journal.pone.0022261
- 出版者・発行元
- PUBLIC LIBRARY SCIENCE
Elucidating the in vitro differentiation of human embryonic stem (ES) and induced pluripotent stem (iPS) cells is important for understanding both normal and pathological hematopoietic development in vivo. For this purpose, a robust and simple hematopoietic differentiation system that can faithfully trace in vivo hematopoiesis is necessary. In this study, we established a novel serum-free monolayer culture that can trace the in vivo hematopoietic pathway from ES/iPS cells to functional definitive blood cells via mesodermal progenitors. Stepwise tuning of exogenous cytokine cocktails induced the hematopoietic mesodermal progenitors via primitive streak cells. These progenitors were then differentiated into various cell lineages depending on the hematopoietic cytokines present. Moreover, single cell deposition assay revealed that common bipotential hemoangiogenic progenitors were induced in our culture. Our system provides a new, robust, and simple method for investigating the mechanisms of mesodermal and hematopoietic differentiation.
- リンク情報
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- DOI
- https://doi.org/10.1371/journal.pone.0022261
- J-GLOBAL
- https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201302247751298870
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/21818303
- PubMed Central
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3144871
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000293282700019&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1371/journal.pone.0022261
- ISSN : 1932-6203
- J-Global ID : 201302247751298870
- PubMed ID : 21818303
- PubMed Central 記事ID : PMC3144871
- Web of Science ID : WOS:000293282700019