論文

査読有り 国際誌
2019年7月12日

Induction of human pluripotent stem cell-derived natural killer cells for immunotherapy under chemically defined conditions.

Biochemical and biophysical research communications
  • Hiroyuki Matsubara
  • ,
  • Akira Niwa
  • ,
  • Tatsutoshi Nakahata
  • ,
  • Megumu K Saito

515
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開始ページ
1
終了ページ
8
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.bbrc.2019.03.085

Natural killer (NK) cells are innate lymphocytes and show cytotoxicity against tumor cells without prior antigen specific stimulation. Because of their innate properties, NK cells are being considered for immunotherapies against various malignancies or leukemia. Human pluripotent stem cells (hPSCs) are capable of inducing enough NK cells for allogeneic transplantation. However, current induction protocols require feeder cells or human or bovine serum for the differentiation and expansion of NK cells, which incurs potential risk for contamination and may cause lot dependency in the cells. To address these issues, here we established a differentiation protocol for developing functional NK cells from hPSCs under a completely chemically-defined condition. The resultant PSC-derived NK cells show comparable phenotypes to those produced under serum-containing condition, exerting strong killing potential against a leukemia cell line in vitro and resistance to tumor growth in vivo. Our protocol can be a useful tool for applying PSC-derived NK cells to future cellular cancer immunotherapies.

リンク情報
DOI
https://doi.org/10.1016/j.bbrc.2019.03.085
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/30948156

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