2013年3月
Wortmannin efficiently suppresses the recovery from radiation-induced damage in pimonidazole-unlabeled quiescent tumor cell population
JOURNAL OF RADIATION RESEARCH
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- 巻
- 54
- 号
- 2
- 開始ページ
- 221
- 終了ページ
- 229
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1093/jrr/rrs094
- 出版者・発行元
- OXFORD UNIV PRESS
Labeling of proliferating (P) cells in mice bearing EL4 tumors was achieved by continuous administration of 5-bromo-2'-deoxyuridine (BrdU). Tumors were irradiated with gamma-rays at 1 h after pimonidazole administration followed by caffeine or wortmannin treatment. Twenty-four hours later, assessment of the responses of quiescent (Q) and total (= P + Q) cell populations were based on the frequencies of micronucleation and apoptosis using immunofluorescence staining for BrdU. The response of the pimonidazole-unlabeled tumor cell fractions was assessed by means of apoptosis frequency using immunofluorescence staining for pimonidazole. The pimonidazole-unlabeled cell fraction showed significantly enhanced radio-sensitivity compared with the whole cell fraction more remarkably in Q cells than total cells. However, a significantly greater decrease in radio-sensitivity in the pimonidazole-unlabeled than the whole cell fraction, evaluated using an assay performed 24 hours after irradiation, was more clearly observed in Q cells than total cells. In both the pimonidazole-unlabeled and the whole cell fractions, wortmannin efficiently suppressed the reduction in sensitivity due to delayed assay. Wortmannin combined with gamma-ray irradiation is useful for suppressing the recovery from radiation-induced damage especially in the pimonidazole-unlabeled cell fraction within the total and Q tumor cell populations.
- リンク情報
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- DOI
- https://doi.org/10.1093/jrr/rrs094
- J-GLOBAL
- https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201302290507353986
- CiNii Articles
- http://ci.nii.ac.jp/naid/120005244224
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/23097299
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000316118000003&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1093/jrr/rrs094
- ISSN : 0449-3060
- eISSN : 1349-9157
- J-Global ID : 201302290507353986
- CiNii Articles ID : 120005244224
- PubMed ID : 23097299
- Web of Science ID : WOS:000316118000003