2013年8月
Identification and Dynamics of Arabidopsis Adaptor Protein-2 Complex and Its Involvement in Floral Organ Development
PLANT CELL
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- 巻
- 25
- 号
- 8
- 開始ページ
- 2958
- 終了ページ
- 2969
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1105/tpc.113.114082
- 出版者・発行元
- AMER SOC PLANT BIOLOGISTS
The adaptor protein-2 (AP-2) complex is a heterotetramer involved in clathrin-mediated endocytosis of cargo proteins from the plasma membrane in animal cells. The homologous genes of AP-2 subunits are present in the genomes of plants; however, their identities and roles in endocytic pathways are not clearly defined in plants. Here, we reveal the molecular composition of the AP-2 complex of Arabidopsis thaliana and its dynamics on the plasma membrane. We identified all of the alpha-, beta-, sigma-, and mu-subunits of the AP-2 complex and detected a weak interaction of the AP-2 complex with clathrin heavy chain. The mu-subunit protein fused to green fluorescent protein (AP2M-GFP) was localized to the plasma membrane and to the cytoplasm. Live-cell imaging using a variable-angle epifluorescence microscope revealed that AP2M-GFP transiently forms punctate structures on the plasma membrane. Homozygous ap2m mutant plants exhibited abnormal floral structures, including reduced stamen elongation and delayed anther dehiscence, which led to a failure of pollination and a subsequent reduction of fertility. Our study provides a molecular basis for understanding AP-2-dependent endocytic pathways in plants and their roles in floral organ development and plant reproduction.
- リンク情報
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- DOI
- https://doi.org/10.1105/tpc.113.114082
- J-GLOBAL
- https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201302278313819135
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/23975897
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000324920500018&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1105/tpc.113.114082
- ISSN : 1040-4651
- eISSN : 1532-298X
- J-Global ID : 201302278313819135
- PubMed ID : 23975897
- Web of Science ID : WOS:000324920500018