論文

査読有り 国際誌
2019年9月

An easy, inexpensive, and sensitive method for the quantification of chitin in insect peritrophic membrane by image processing.

Bioscience, biotechnology, and biochemistry
  • Ryu Nakata
  • ,
  • Naoko Yoshinaga
  • ,
  • Masayoshi Teraishi
  • ,
  • Yutaka Okumoto
  • ,
  • Naoki Mori

83
9
開始ページ
1624
終了ページ
1629
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1080/09168451.2019.1611407

Chitin, poly (β-(1→4)-N-acetyl-d-glucosamine), is an important biopolymer for insects that is utilized as a major component of peritrophic membrane. The chitin content in peritrophic membrane is of expedient interest from a pest control perspective, although it is hard to quantify chitin. In this study, we establish a facile method for the quantification of chitin in peritrophic membrane by image processing. In this method, chitin was indirectly quantified using chitosan-I3- complex, which exhibited a specific red-purple color. A calibration curve using a chitosan solution showed good linearity in a concentration range of 0.05-0.5 μg/μL. We quantified the amount of chitin in peritrophic membrane of Spodoptera litura (Lepidoptera: Noctuidae) larvae using this method. Throughout the study, only common inexpensive regents and easily attainable apparatuses were employed. This method can be easily applied to the sensitive quantification of the amounts of chitin and chitosan in materials by wide range of researchers. Abbreviations: LOD: limit of detection; LOQ: limit of quantification; ROI: region of interest; RSD: relative standard deviation.

リンク情報
DOI
https://doi.org/10.1080/09168451.2019.1611407
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/31050316
URL
https://www.tandfonline.com/doi/full/10.1080/09168451.2019.1611407

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