論文

査読有り 国際誌
2014年7月4日

Insect-induced daidzein, formononetin and their conjugates in soybean leaves.

Metabolites
  • Shinichiro Murakami
  • ,
  • Ryu Nakata
  • ,
  • Takako Aboshi
  • ,
  • Naoko Yoshinaga
  • ,
  • Masayoshi Teraishi
  • ,
  • Yutaka Okumoto
  • ,
  • Atsushi Ishihara
  • ,
  • Hironobu Morisaka
  • ,
  • Alisa Huffaker
  • ,
  • Eric A Schmelz
  • ,
  • Naoki Mori

4
3
開始ページ
532
終了ページ
46
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.3390/metabo4030532

In response to attack by bacterial pathogens, soybean (Gylcine max) leaves accumulate isoflavone aglucones, isoflavone glucosides, and glyceollins. In contrast to pathogens, the dynamics of related insect-inducible metabolites in soybean leaves remain poorly understood. In this study, we analyzed the biochemical responses of soybean leaves to Spodoptera litura (Lepidoptera: Noctuidae) herbivory and also S. litura gut contents, which contain oral secretion elicitors. Following S. litura herbivory, soybean leaves displayed an induced accumulation of the flavone and isoflavone aglycones 4',7-dihyroxyflavone, daidzein, and formononetin, and also the isoflavone glucoside daidzin. Interestingly, foliar application of S. litura oral secretions also elicited the accumulation of isoflavone aglycones (daidzein and formononetin), isoflavone 7-O-glucosides (daidzin, ononin), and isoflavone 7-O-(6'-O-malonyl-β-glucosides) (malonyldaidzin, malonylononin). Consistent with the up-regulation of the isoflavonoid biosynthetic pathway, folair phenylalanine levels also increased following oral secretion treatment. To establish that these metabolitic changes were the result of de novo biosynthesis, we demonstrated that labeled (13C9) phenylalanine was incorporated into the isoflavone aglucones. These results are consistent with the presence of soybean defense elicitors in S. litura oral secretions. We demonstrate that isoflavone aglycones and isoflavone conjugates are induced in soybean leaves, not only by pathogens as previously demonstrated, but also by foliar insect herbivory.

リンク情報
DOI
https://doi.org/10.3390/metabo4030532
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/25000357
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4192678

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