論文

査読有り
2008年11月

Active role of fatty acid amino acid conjugates in nitrogen metabolism in Spodoptera litura larvae

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
  • Naoko Yoshinaga
  • ,
  • Takako Aboshi
  • ,
  • Hiroaki Abe
  • ,
  • Ritsuo Nishida
  • ,
  • Hans T. Alborn
  • ,
  • James H. Tumlinson
  • ,
  • Naoki Mori

105
46
開始ページ
18058
終了ページ
18063
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1073/pnas.0809623105
出版者・発行元
NATL ACAD SCIENCES

Since the first fatty acid amino acid conjugate (FAC) was isolated from regurgitant of Spodoptera exigua larvae in 1997 [volicitin: N-(17-hydroxylinolenoyl)-L-glutamine], their role as elicitors of induced responses in plants has been well documented. However, studies of the biosyntheses and the physiological role of FACs in the insect have been minimal. By using (14)C-labeled glutamine, glutamic acid, and linolenic acid in feeding studies of Spodoptera litura larvae, combined with tissue analyses, we found glutamine in the midgut cells to be a major source for biosynthesis of FACs. Furthermore, 20% of the glutamine moiety of FACs was derived from glutamic acid and ammonia through enzymatic reaction of glutamine synthetase (GS). To determine whether FACs improve GS productivity, we studied nitrogen assimilation efficiency of S. litura larvae fed on artificial diets containing (15)NH(4)Cl and glutamic acid. When the diet was enriched with linolenic acid, the nitrogen assimilation efficiency improved from 40% to >60%. In the lumen, the biosynthesized FACs are hydrolyzed to fatty acids and glutamine, which are reabsorbed into tissues and hemolymph. These results strongly suggested that FACs play an active role in nitrogen assimilation in Lepidoptera larva and that glutamine containing FACs in the gut lumen may function as a form of storage of glutamine, a key compound of nitrogen metabolism.

Web of Science ® 被引用回数 : 37

リンク情報
DOI
https://doi.org/10.1073/pnas.0809623105
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/18997016
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000261225600088&DestApp=WOS_CPL

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