論文

査読有り
2010年8月

Self-assembly immobilization of hyaluronan thiosemicarbazone on a gold surface for cell culture applications

CARBOHYDRATE POLYMERS
  • Nobuo Tanaka
  • ,
  • Yuka Yoshiike
  • ,
  • Chiharu Yoshiyama
  • ,
  • Takuya Kitaoka

82
1
開始ページ
100
終了ページ
105
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.carbpol.2010.04.028
出版者・発行元
ELSEVIER SCI LTD

Hyaluronan (HA)-fixed layers were successfully prepared on a gold coated glass plate via self-assembly chemisorption of HA derivatives whose aldehydic reducing ends were selectively modified with thiosemicarbazide (TSC). Quartz crystal microbalance analysis and X-ray photoelectron spectroscopy suggested formation of the HA layers on the gold surfaces through spontaneous S-Au interaction, resulting in stability in aqueous culture medium. Biological functions of the HA layers were investigated by cell adhesion assay using mouse fibroblast cells (NIH-3T3). Cell attachment to the hydrophilic HA surface (water contact angle approximate to 11 degrees) was far superior to cell attachment to a sugar-free, hydrophobic gold substrate (approximate to 57 degrees), while a spin-coated film composed of water-soluble, TSC-free HA molecules made little contribution to cell adhesion. After 48 h incubation, the NIH-3T3 cells proliferated well only on the HA layers and retained their original viability. This novel approach to surface glyco-modification with biocompatible HA is expected to have potential applications as a biofunctional scaffold for cell culture applications. (C) 2010 Elsevier Ltd. All rights reserved.

リンク情報
DOI
https://doi.org/10.1016/j.carbpol.2010.04.028
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000280574400015&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.carbpol.2010.04.028
  • ISSN : 0144-8617
  • Web of Science ID : WOS:000280574400015

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