2010年8月
Self-assembly immobilization of hyaluronan thiosemicarbazone on a gold surface for cell culture applications
CARBOHYDRATE POLYMERS
- ,
- ,
- ,
- 巻
- 82
- 号
- 1
- 開始ページ
- 100
- 終了ページ
- 105
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.carbpol.2010.04.028
- 出版者・発行元
- ELSEVIER SCI LTD
Hyaluronan (HA)-fixed layers were successfully prepared on a gold coated glass plate via self-assembly chemisorption of HA derivatives whose aldehydic reducing ends were selectively modified with thiosemicarbazide (TSC). Quartz crystal microbalance analysis and X-ray photoelectron spectroscopy suggested formation of the HA layers on the gold surfaces through spontaneous S-Au interaction, resulting in stability in aqueous culture medium. Biological functions of the HA layers were investigated by cell adhesion assay using mouse fibroblast cells (NIH-3T3). Cell attachment to the hydrophilic HA surface (water contact angle approximate to 11 degrees) was far superior to cell attachment to a sugar-free, hydrophobic gold substrate (approximate to 57 degrees), while a spin-coated film composed of water-soluble, TSC-free HA molecules made little contribution to cell adhesion. After 48 h incubation, the NIH-3T3 cells proliferated well only on the HA layers and retained their original viability. This novel approach to surface glyco-modification with biocompatible HA is expected to have potential applications as a biofunctional scaffold for cell culture applications. (C) 2010 Elsevier Ltd. All rights reserved.
- リンク情報
- ID情報
-
- DOI : 10.1016/j.carbpol.2010.04.028
- ISSN : 0144-8617
- Web of Science ID : WOS:000280574400015