2019年8月
Exosome surface glycans reflect osteogenic differentiation of mesenchymal stem cells: Profiling by an evanescent field fluorescence-assisted lectin array system
SCIENTIFIC REPORTS
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- ,
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- 巻
- 9
- 号
- 1
- 開始ページ
- 11497
- 終了ページ
- 11497
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1038/s41598-019-47760-x
- 出版者・発行元
- NATURE PUBLISHING GROUP
Extracellular vesicles (EVs) carry information between cells in the form of biomolecules. Such molecules have been found to serve as biomarkers. Glycans attached to surface molecules on EVs are involved in their cellular uptake. In this study, we examined glycan profiles of small EVs which are generally termed exosomes before and after osteogenic differentiation of adipose-derived mesenchymal stem cells (MSCs) by an evanescent field fluorescence-assisted (EFF)-lectin array system to discover glycan biomarkers for osteogenic differentiation. We found few differences between exosomes before and after osteogenic differentiation of MSCs in terms of fundamental characteristics such as size, morphology, and exosomal marker proteins. However, specific lectins bound strongly to exosomes from differentiated cells. Exosomes from osteogenically differentiated MSCs bound strongly to fucose- and mannose-binding lectins, especially at a high concentration of exosomes. In summary, we found that several lectins bound to exosomes from differentiated MSCs more strongly than to those from undifferentiated cells using an EFF-lectin array system, indicating that monitoring exosomal surface glycans may identify predictive indexes of osteogenic differentiation.
- リンク情報
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- DOI
- https://doi.org/10.1038/s41598-019-47760-x
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/31395910
- PubMed Central
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6687741
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000479187800003&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1038/s41598-019-47760-x
- ISSN : 2045-2322
- PubMed ID : 31395910
- PubMed Central 記事ID : PMC6687741
- Web of Science ID : WOS:000479187800003