論文

査読有り
2014年10月

Induction of Primordial Germ Cell-Like Cells From Mouse Embryonic Stem Cells by ERK Signal Inhibition

STEM CELLS
  • Tohru Kimura
  • Yoshiaki Kaga
  • Hiroshi Ohta
  • Mika Odamoto
  • Yoichi Sekita
  • Kunpeng Li
  • Noriko Yamano
  • Keita Fujikawa
  • Ayako Isotani
  • Norihiko Sasaki
  • Masashi Toyoda
  • Katsuhiko Hayashi
  • Masaru Okabe
  • Takashi Shinohara
  • Mitinori Saitou
  • Toru Nakano
  • 全て表示

32
10
開始ページ
2668
終了ページ
2678
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1002/stem.1781
出版者・発行元
WILEY-BLACKWELL

Primordial germ cells (PGCs) are embryonic germ cell precursors. Specification of PGCs occurs under the influence of mesodermal induction signaling during in vivo gastrulation. Although bone morphogenetic proteins and Wnt signaling play pivotal roles in both mesodermal and PGC specification, the signal regulating PGC specification remains unknown. Coculture of mouse embryonic stem cells (ESCs) with OP9 feeder cells induces mesodermal differentiation in vitro. Using this mesodermal differentiation system, we demonstrated that PGC-like cells were efficiently induced from mouse ESCs by extracellular signal-regulated kinase (ERK) signaling inhibition. Inhibition of ERK signaling by a MAPK/ERK kinase (MEK) inhibitor upregulated germ cell marker genes but downregulated mesodermal genes. In addition, the PGC-like cells showed downregulation of DNA methylation and formed pluripotent stem cell colonies upon treatment with retinoic acid. These results show that inhibition of ERK signaling suppresses mesodermal differentiation but activates germline differentiation program in this mesodermal differentiation system. Our findings provide a new insight into the signaling networks regulating PGC specification.

リンク情報
DOI
https://doi.org/10.1002/stem.1781
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/24989326
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000342359400010&DestApp=WOS_CPL
ID情報
  • DOI : 10.1002/stem.1781
  • ISSN : 1066-5099
  • eISSN : 1549-4918
  • PubMed ID : 24989326
  • Web of Science ID : WOS:000342359400010

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