2006年3月
Normal specification of the extraembryonic lineage after somatic nuclear transfer
FEBS LETTERS
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- 巻
- 580
- 号
- 7
- 開始ページ
- 1801
- 終了ページ
- 1806
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.febslet.2006.02.036
- 出版者・発行元
- ELSEVIER SCIENCE BV
To examine the establishment and maintenance of trophectoderm (TE) lineage in somatic cloned blastocysts, the expression of Cdx2, a key molecule for specification of TE fate, was immunohistochemically examined simultaneously with Oct4 expression. Cloned mouse embryos were made by nuclear transfer using cumulus cells, tail-tip fibroblasts, and embryonic stem cells. After 96 h of culture, the rates of Oct4-expressmg blastocysts were as low as 50%, and 60% for cumulus and fibroblast clones, respectively. However, regardless of Oct4 expression, the majority of those cloned blastocysts (> 90%) normally expressed Cdx2. Thus, even though somatic cloned embryos have reduced potential to produce the inner cell mass lineage, the TE lineage can be established and maintained. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
- リンク情報
- ID情報
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- DOI : 10.1016/j.febslet.2006.02.036
- ISSN : 0014-5793
- PubMed ID : 16513118
- Web of Science ID : WOS:000236338300018