論文

査読有り
2005年1月

Propagation of an infertile hermaphrodite mouse lacking germ cells by using nuclear transfer and embryonic stem cell technology

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
  • S Wakayama
  • ,
  • S Kishigami
  • ,
  • N Van Thuan
  • ,
  • H Ohta
  • ,
  • T Hikichi
  • ,
  • E Mizutani
  • ,
  • R Yanagimachi
  • ,
  • T Wakayama

102
1
開始ページ
29
終了ページ
33
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1073/pnas.0408548102
出版者・発行元
NATL ACAD SCIENCES

Animals generated by systematic mutagenesis and routine breeding are often infertile because they lack germ cells, and maintenance of such lines of animals has been impossible. We found a hermaphrodite infertile mouse in our colony, a genetic male with an abnormal Y chromosome lacking developing germ cells. We tried to clone this mouse by conventional nuclear transfer but without success. ES cells produced from blastocysts, which had been cloned by using somatic cell nuclear transfer (ntES cells) from this mouse, were also unable to produce offspring when injected into enucleated oocytes. Although we were able to produce two chimeric offspring using these ntES cells by tetraploid complementation, they were infertile, because they also lacked developing germ cells. However, when such ntES cells were injected into normal diploid blastocysts, many chimeric offspring were produced. One such male offspring transmitted hermaphrodite mouse genes to fertile daughters via X chromosome-bearing sperm. Thus, ntES cells were used to propagate offspring from infertile mice lacking germ cells.

リンク情報
DOI
https://doi.org/10.1073/pnas.0408548102
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/15618395
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000226216400007&DestApp=WOS_CPL
ID情報
  • DOI : 10.1073/pnas.0408548102
  • ISSN : 0027-8424
  • PubMed ID : 15618395
  • Web of Science ID : WOS:000226216400007

エクスポート
BibTeX RIS