論文

査読有り 国際誌
2021年8月5日

PRDX1 is essential for the viability and maintenance of reactive oxygen species in chicken DT40.

Genes and environment : the official journal of the Japanese Environmental Mutagen Society
  • Takahito Moriwaki
  • ,
  • Akari Yoshimura
  • ,
  • Yuki Tamari
  • ,
  • Hiroyuki Sasanuma
  • ,
  • Shunichi Takeda
  • ,
  • Masayuki Seki
  • ,
  • Keizo Tano

43
1
開始ページ
35
終了ページ
35
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1186/s41021-021-00211-4

BACKGROUND: Peroxiredoxin 1 (PRDX1) is a member of a ubiquitous family of thiol peroxidases that catalyze the reduction of peroxides, including hydrogen peroxide. It functions as an antioxidant enzyme, similar to catalase and glutathione peroxidase. PRDX1 was recently shown act as a sensor of reactive oxygen species (ROS) and play a role in ROS-dependent intracellular signaling pathways. To investigate its physiological functions, PRDX1 was conditionally disrupted in chicken DT40 cells in the present study. RESULTS: The depletion of PRDX1 resulted in cell death with increased levels of intracellular ROS. PRDX1-depleted cells did not show the accumulation of chromosomal breaks or sister chromatid exchange (SCE). These results suggest that cell death in PRDX1-depleted cells was not due to DNA damage. 2-Mercaptoethanol protected against cell death in PRDX1-depleted cells and also suppressed elevations in ROS. CONCLUSIONS: PRDX1 is essential in chicken DT40 cells and plays an important role in maintaining intracellular ROS homeostasis (or in the fine-tuning of cellular ROS levels). Cells deficient in PRDX1 may be used as an endogenously deregulated ROS model to elucidate the physiological roles of ROS in maintaining proper cell growth.

リンク情報
DOI
https://doi.org/10.1186/s41021-021-00211-4
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/34353368
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8340460
ID情報
  • DOI : 10.1186/s41021-021-00211-4
  • PubMed ID : 34353368
  • PubMed Central 記事ID : PMC8340460

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