論文

査読有り
2017年12月

In Situ Nondestructive Analysis of Kalanchoe pinnata Leaf Surface Structure by Polarization-Modulation Infrared Reflection Absorption Spectroscopy

JOURNAL OF PHYSICAL CHEMISTRY B
  • Tetsuya Hama
  • ,
  • Akira Kouchi
  • ,
  • Naoki Watanabe
  • ,
  • Shinichi Enami
  • ,
  • Takafumi Shimoaka
  • ,
  • Takeshi Hasegawa

121
49
開始ページ
11124
終了ページ
11131
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1021/acs.jpcb.7b09173
出版者・発行元
AMER CHEMICAL SOC

The outermost surface of the leaves of land plants is covered with a lipid membrane called the cuticle that protects against various stress factors. Probing the molecular-level structure of the intact cuticle is highly desirable for understanding its multifunctional properties. We report the in situ characterization of the surface structure of Kalanchoe pinnata leaves using polarization-modulation infrared reflection absorption spectroscopy (PM-IRRAS). Without sample pretreatment, PM-IRRAS measures the IR spectra of the leaf cuticle of a potted K. pinnata plant. The peak position of the CH2-related modes shows that the cuticular waxes on the leaf surface are mainly crystalline, and the alkyl chains are highly packed in an all-trans zigzag conformation. The surface selection rule of PM-IRRAS revealed the average orientation of the cuticular molecules, as indicated by the positive and negative signals of the IR peaks. This unique property of PM-IRRAS revealed that the alkyl chains of the waxes and the main chains of polysaccharides are oriented almost perpendicular to the leaf surface. The nondestructive, background-free, and environmental gas-free nature of PM-IRRAS allows the structure and chemistry of the leaf cuticle to be studied directly in its native environment.

リンク情報
DOI
https://doi.org/10.1021/acs.jpcb.7b09173
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/29148773
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000418394100011&DestApp=WOS_CPL
ID情報
  • DOI : 10.1021/acs.jpcb.7b09173
  • ISSN : 1520-6106
  • eISSN : 1520-5207
  • PubMed ID : 29148773
  • Web of Science ID : WOS:000418394100011

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