2010年3月16日
Temperature-sensitive reaction of a photosensor protein YcgF: possibility of a role of temperature sensor.
Biochemistry
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- ,
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- 巻
- 49
- 号
- 10
- 開始ページ
- 2288
- 終了ページ
- 96
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1021/bi902121z
- 出版者・発行元
- AMER CHEMICAL SOC
The spectrally silent photoreaction of a blue light sensor protein YcgF, composed of the N-terminal BLUF domain and the C-terminal EAL domain, was investigated by the time-resolved transient grating method. Comparing photoinduced reactions of full-length YcgF with that of the BLUF-linker Construct, it was found that a major conformation change after photoinduced dimerization is predominantly localized on the EAL domain, Furthermore, the photoinduced conformational change displayed significant temperature dependence. Tills result is explained by an equilibrium of reactive and nonreactive YcgF species, with the population of photoreactive species decreasing as the temperature is lowered in the dark state. We consider that the dimer form is the nonreactive species and it is the dominant species at lower temperatures. The temperature sensitivity of the photoreaction of YcgF suggests that this protein could have a biological function as a temperature sensor as well its behaving as a light sensor.
- リンク情報
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- DOI
- https://doi.org/10.1021/bi902121z
- J-GLOBAL
- https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201002204251531932
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/20141167
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000275227000021&DestApp=WOS_CPL
- ID情報
-
- DOI : 10.1021/bi902121z
- ISSN : 0006-2960
- J-Global ID : 201002204251531932
- PubMed ID : 20141167
- Web of Science ID : WOS:000275227000021