2010年12月1日
Kinetics of conformational changes of the FKF1-LOV domain upon photoexcitation.
Biophysical journal
- ,
- ,
- ,
- 巻
- 99
- 号
- 11
- 開始ページ
- 3831
- 終了ページ
- 9
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.bpj.2010.10.005
- 出版者・発行元
- CELL PRESS
The photochemical reaction dynamics of a light oxygen voltage (LOV) domain from the blue light sensor protein FKF1 (flavin binding Kelch repeat F box) was studied by means of the pulsed laser induced transient grating method The observed absorption spectral changes upon photoexcitation were similar to the spectral changes observed for typical LOV domain proteins (e g, phototropins) The adduct formation took place with a time constant of 6 As After this reaction, a significant conformational change with a time constant of 6 ms was observed as a change in the diffusion coefficient An FKF1 LOV mutant without the conserved loop connecting helices E and F which is present only in the FKF1/LOV Kelch protein 2/ZEITLUPE family did not show these slow phase dynamics This result indicates that the conformational change in the loop region represents a major change in the FKF1 LOV photoreaction
- リンク情報
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- DOI
- https://doi.org/10.1016/j.bpj.2010.10.005
- CiNii Articles
- http://ci.nii.ac.jp/naid/120002709547
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/21112308
- PubMed Central
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2998605
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000285033800036&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1016/j.bpj.2010.10.005
- ISSN : 0006-3495
- eISSN : 1542-0086
- CiNii Articles ID : 120002709547
- PubMed ID : 21112308
- PubMed Central 記事ID : PMC2998605
- Web of Science ID : WOS:000285033800036