2007年10月
Cross-primed CD8(+) cytotoxic T cells induce severe helicobacter-associated gastritis in the absence of CD4(+) T cells
HELICOBACTER
- 巻
- 12
- 号
- 5
- 開始ページ
- 486
- 終了ページ
- 497
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1111/j.1523-5378.2007.00536.x
- 出版者・発行元
- WILEY
Background: Although previous studies have reported important roles of CD4(+) type1-helper T cells and regulatory T cells in Helicobacter-associated gastritis, the significance of CD8(+) cytotoxic T cells remains unknown. To study the roles of CD8(+) T cells, we examined the immune response in the gastric mucosa of Helicobacter felis-infected major histocompatibility complex (MHC) class II-deficient (II-/-) mice, which lack CD4(+) T cells.
Materials and methods: Stomachs from H. felis-infected wild-type and infected MHC II-/- mice were examined histologically and immunohistochemically. Gastric acidity and serum levels of anti-H. felis antibodies were measured. The expression of pro-inflammatory and anti-inflammatory cytokine, Fas-ligand, perforin, and Foxp3 genes in the gastric mucosa was investigated.
Results: H. felis-infected MHC II-/- mice developed severe gastritis, accompanied by marked infiltration of CD8(+) cells. At 1 and 2 months after inoculation, mucosal inflammation and atrophy were more severe in MHC II-/- mice, although gastritis had reached similar advanced stages at 3 months after inoculation. There was little infiltration of CD4(+) cells, and no Foxp3-positive cells were detected in the gastric mucosa of the infected MHC II-/- mice. The expression of the interleukin-1 beta and Fas-ligand genes was up regulated, but that of Foxp3 was down regulated in the infected MHC II-/- mice. Serum levels of anti-H. felis antibodies were lower in the infected MHC II-/- mice, despite severe gastritis.
Conclusions: The present study suggests that cross-primed CD8(+) cytotoxic T cells can induce severe H.-associated gastritis in the absence of CD4(+) helper T cells and that Foxp3-positive cells may have an important role in the control of gastric inflammation.
Materials and methods: Stomachs from H. felis-infected wild-type and infected MHC II-/- mice were examined histologically and immunohistochemically. Gastric acidity and serum levels of anti-H. felis antibodies were measured. The expression of pro-inflammatory and anti-inflammatory cytokine, Fas-ligand, perforin, and Foxp3 genes in the gastric mucosa was investigated.
Results: H. felis-infected MHC II-/- mice developed severe gastritis, accompanied by marked infiltration of CD8(+) cells. At 1 and 2 months after inoculation, mucosal inflammation and atrophy were more severe in MHC II-/- mice, although gastritis had reached similar advanced stages at 3 months after inoculation. There was little infiltration of CD4(+) cells, and no Foxp3-positive cells were detected in the gastric mucosa of the infected MHC II-/- mice. The expression of the interleukin-1 beta and Fas-ligand genes was up regulated, but that of Foxp3 was down regulated in the infected MHC II-/- mice. Serum levels of anti-H. felis antibodies were lower in the infected MHC II-/- mice, despite severe gastritis.
Conclusions: The present study suggests that cross-primed CD8(+) cytotoxic T cells can induce severe H.-associated gastritis in the absence of CD4(+) helper T cells and that Foxp3-positive cells may have an important role in the control of gastric inflammation.
- リンク情報
- ID情報
-
- DOI : 10.1111/j.1523-5378.2007.00536.x
- ISSN : 1083-4389
- eISSN : 1523-5378
- PubMed ID : 17760716
- Web of Science ID : WOS:000248996100002