論文

査読有り
2014年8月

Preclinical Validation of Talaporfin Sodium-Mediated Photodynamic Therapy for Esophageal Squamous Cell Carcinoma

PLOS ONE
  • Shinya Ohashi
  • ,
  • Osamu Kikuchi
  • ,
  • Mihoko Tsurumaki
  • ,
  • Yukie Nakai
  • ,
  • Hiroi Kasai
  • ,
  • Takahiro Horimatsu
  • ,
  • Shin'ichi Miyamoto
  • ,
  • Akira Shimizu
  • ,
  • Tsutomu Chiba
  • ,
  • Manabu Muto

9
8
開始ページ
e103126
終了ページ
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1371/journal.pone.0103126
出版者・発行元
PUBLIC LIBRARY SCIENCE

Photodynamic therapy (PDT) kills cancer cells via a photochemical reaction mediated by an oncotropic photosensitizer. Herein, we performed an experimental preclinical study to validate the anti-tumour effect of talaporfin sodium-mediated PDT (t-PDT) for esophageal squamous cell carcinoma (ESCC) cells. We used human ESCC cells derived from various differentiation grades or resistant to 5-fluorouracil (5-FU). The cytotoxic effect of t-PDT was determined by evaluating cell viability, apoptosis and generation of reactive oxygen species (ROS) and DNA double-strand breaks. Furthermore, the antitumour effect of t-PDT was assessed using an anchorage-independent cell-growth assay and xenograft transplantation models. t-PDT induced potent cytotoxicity in ESCC cells independent of their differentiation grade or 5-FU resistance. Moreover, t-PDT induced robust apoptosis, as indicated by cell shrinkage, perinuclear vacuolization, nuclear fragmentation and induction of annexin V-positive cells. This apoptotic response was accompanied by concurrent activation of ROS, and induction of DNA double-strand breakage. Importantly, t-PDT suppressed efficiently anchorage-independent cell growth as well as ESCC-xenografted tumor formation. In aggregate, t-PDT showed anti-tumor potential for ESCC cells with various histological grades or chemoresistance, providing a novel translational rationale of t-PDT for the treatment of ESCC.

リンク情報
DOI
https://doi.org/10.1371/journal.pone.0103126
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000339812700017&DestApp=WOS_CPL
ID情報
  • DOI : 10.1371/journal.pone.0103126
  • ISSN : 1932-6203
  • Web of Science ID : WOS:000339812700017

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