2013年9月
The Effectiveness and Limitations of Triphenyltetrazolium Chloride to Detect Acute Myocardial Infarction at Forensic Autopsy
AMERICAN JOURNAL OF FORENSIC MEDICINE AND PATHOLOGY
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- 巻
- 34
- 号
- 3
- 開始ページ
- 242
- 終了ページ
- 247
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1097/PAF.0b013e31828879cd
- 出版者・発行元
- LIPPINCOTT WILLIAMS & WILKINS
Triphenyltetrazolium chloride (TTC) is one of the most conventional stains to detect infarcted area of the heart in animal experiments. However, its availability and limitations have not been thoroughly discussed in the forensic field. Here, authors stained human hearts with TTC soon after the harvest. Photographs of the samples were analyzed using image analysis software, which evaluated the occupying ratio of the stained area on the surface of each slice. The results showed that the stainability of TTC declines with the length of the postmortem interval (PMI). Specimens reacted well to TTC within 1.5 days after death and then decreased the stainability logarithmically with PMI (y = -0.294 In (x) + 1.0441; x = PMI, y = TTC-stained area / total myocardial area, R-2 = 0.5673). Samples with old myocardial infarction produced clear TTC contrast; normal tissue is vivid red, and fibrotic myocardium is white discoloration. In acute myocardial infarction cases where death occurred within 9 hours after the attack, however, the detection of infarcted area was very difficult even when PMI was less than 1.5 days. In summary, the TTC method may be useful within 1.5 days after death, but short suffering period before death disturbs its staining efficiency.
- リンク情報
- ID情報
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- DOI : 10.1097/PAF.0b013e31828879cd
- ISSN : 0195-7910
- eISSN : 1533-404X
- PubMed ID : 23949140
- Web of Science ID : WOS:000330352500016