MISC

査読有り
2006年7月

Functional uncoupling between Ca2+ release and afterhyperpolarization in mutant hippocampal neurons lacking junctophilins

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
  • Shigeki Moriguchi
  • Miyuki Nishi
  • Shinji Komazaki
  • Hiroyuki Sakagami
  • Taisuke Miyazaki
  • Haruko Masumiya
  • Shin-ya Saito
  • Masahiko Watanabe
  • Hisatake Kondo
  • Hiromu Yawo
  • Kohji Fukunaga
  • Hiroshi Takeshima
  • 全て表示

103
28
開始ページ
10811
終了ページ
10816
記述言語
英語
掲載種別
DOI
10.1073/pnas.0509863103
出版者・発行元
NATL ACAD SCIENCES

Junctional membrane complexes (JMCs) composed of the plasma membrane and endoplasmic/sarcoplasmic reticulum seem to be a structural platform for channel crosstalk. Junctophilins (JPs) contribute to JMC formation by spanning the sarcoplasmic reticulum membrane and binding with the plasma membrane in muscle cells. In this article, we report that mutant JP double-knockout (JP-DKO) mice lacking neural JP subtypes exhibited an irregular hindlimb reflex and impaired memory. Electrophysiological experiments indicated that the activation of small-conductance Ca2+-activated K+ channels responsible for afterhyperpolarization in hippocampal neurons requires endoplasmic reticulum Ca2+ release through ryanodine receptors, triggered by NMDA receptor-mediated Ca2+ influx. We propose that in JP-DKO neurons lacking afterhyperpolarization, the functional communications between NMDA receptors, ryanodine receptors, and small-conductance Ca2+-activated K+ channels are disconnected because of JMC disassembly. Moreover, JP-DKO neurons showed an impaired long-term potentiation and hyperactivation of Ca2+/calmodulin-dependent protein kinase II. Therefore, JPs seem to have an essential role in neural excitability fundamental to plasticity and integrated functions.

リンク情報
DOI
https://doi.org/10.1073/pnas.0509863103
J-GLOBAL
https://jglobal.jst.go.jp/detail?JGLOBAL_ID=200902254561367950
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000239047400049&DestApp=WOS_CPL
ID情報
  • DOI : 10.1073/pnas.0509863103
  • ISSN : 0027-8424
  • J-Global ID : 200902254561367950
  • Web of Science ID : WOS:000239047400049

エクスポート
BibTeX RIS