Yohei Hizukuri

Profile Information

Affiliation: Institute for Life and Medical Sciences, Kyoto University

Degree: 博士（理学）(Mar, 2009, 名古屋大学大学院理学研究科)

ORCID ID: https://orcid.org/0000-0003-0594-1160
J-GLOBAL ID: 201401070633532105
researchmap Member ID: 7000009470

Research Areas

Papers

S2P intramembrane protease RseP degrades small membrane proteins and suppresses the cytotoxicity of intrinsic toxin HokB

Tatsuhiko Yokoyama, Yutaro Yamagata, Saisei Honna, Shinya Mizuno, Shizuka Katagiri, Rika Oi, Terukazu Nogi, Yohei Hizukuri, Yoshinori Akiyama

mBio, 14(4) e0108623, Aug 31, 2023 Peer-reviewedCorresponding author
Mechanistic insights into intramembrane proteolysis by E. coli site-2 protease homolog RseP.

Yuki Imaizumi, Kazunori Takanuki, Takuya Miyake, Mizuki Takemoto, Kunio Hirata, Mika Hirose, Rika Oi, Tatsuya Kobayashi, Kenichi Miyoshi, Rie Aruga …

Science advances, 8(34) eabp9011, Aug 26, 2022 Peer-reviewedCorresponding author
Mechanistic insights into intramembrane proteolysis by E. coli site-2 protease homolog RseP

Yuki Imaizumi, Kazunori Takanuki, Takuya Miyake, Mizuki Takemoto, Kunio Hirata, Mika Hirose, Rika Oi, Tatsuya Kobayashi, Kenichi Miyoshi, Rie Aruga …

bioRxiv, Jan 31, 2022 Corresponding author
Moving toward generalizable NZ-1 labeling for 3D structure determination with optimized epitope-tag insertion.

Risako Tamura-Sakaguchi, Rie Aruga, Mika Hirose, Toru Ekimoto, Takuya Miyake, Yohei Hizukuri, Rika Oi, Mika K Kaneko, Yukinari Kato, Yoshinori Akiyama …

Acta crystallographica. Section D, Structural biology, 77(Pt 5) 645-662, May 1, 2021 Peer-reviewed
The Escherichia coli S2P intramembrane protease RseP regulates ferric citrate uptake by cleaving the sigma factor regulator FecR.

Tatsuhiko Yokoyama, Tomoya Niinae, Kazuya Tsumagari, Koshi Imami, Yasushi Ishihama, Yohei Hizukuri, Yoshinori Akiyama

The Journal of biological chemistry, 296 100673-100673, Apr 15, 2021 Peer-reviewedCorresponding author
Escherichia coli S2P family intramembrane protease RseP is engaged in the regulated sequential cleavages of FecR in the ferric citrate signaling

Tatsuhiko Yokoyama, Tomoya Niinae, Kazuya Tsumagari, Koshi Imami, Yasushi Ishihama, Yohei Hizukuri, Yoshinori Akiyama

bioRxiv, Feb 4, 2021 Corresponding author
Involvement of a Membrane-Bound Amphiphilic Helix in Substrate Discrimination and Binding by an Escherichia coli S2P Peptidase RseP.

Takuya Miyake, Yohei Hizukuri, Yoshinori Akiyama

Frontiers in microbiology, 11 607381-607381, Nov 27, 2020 Peer-reviewedCorresponding author
Reversible autoinhibitory regulation of Escherichia coli metallopeptidase BepA for selective β-barrel protein degradation.

Yasushi Daimon, Shin-Ichiro Narita, Ryoji Miyazaki, Yohei Hizukuri, Hiroyuki Mori, Yoshiki Tanaka, Tomoya Tsukazaki, Yoshinori Akiyama

Proceedings of the National Academy of Sciences of the United States of America, 117(45) 27989-27996, Nov 10, 2020 Peer-reviewed
An in vivo protease activity assay for investigating the functions of the Escherichia coli membrane protease HtpX.

Yoshitani K, Hizukuri Y, Akiyama Y

FEBS letters, 593(8) 842-851, Apr, 2019 Peer-reviewed
Endosomal rab cycles regulate parkin-mediated mitophagy

Koji Yamano, Chunxin Wang, Shireen A. Sarraf, Christian Münch, Reika Kikuchi, Nobuo N. Noda, Yohei Hizukuri, Masato T. Kanemaki, Wade Harper, Keiji Tanaka …

eLife, 7 e31326, Jan 23, 2018 Peer-reviewed
Involvement of a conserved GFG motif region in substrate binding by RseP, an Escherichia coli S2P protease

Koichiro Akiyama, Yohei Hizukuri, Yoshinori Akiyama

Molecular Microbiology, 104(5) 737-751, Jun, 2017 Peer-reviewed
Biochemical Characterization of Function and Structure of RseP, an Escherichia coli S2P Protease

Y. Hizukuri, K. Akiyama, Y. Akiyama

Methods in Enzymology, 584 1-33, 2017
Roles of the membrane-reentrant β-hairpin-like loop of RseP protease in selective substrate cleavage

Koichiro Akiyama, Shinya Mizuno, Yohei Hizukuri, Hiroyuki Mori, Terukazu Nogi, Yoshinori Akiyama

eLife, 4 e08928, Oct, 2015 Peer-reviewed
A Structure-Based Model of Substrate Discrimination by a Noncanonical PDZ Tandem in the Intramembrane-Cleaving Protease RseP

Yohei Hizukuri, Takashi Oda, Sanae Tabata, Keiko Tamura-Kawakami, Rika Oi, Mamoru Sato, Junichi Takagi, Yoshinori Akiyama, Terukazu Nogi

Structure, 22(2) 326-336, Feb, 2014 Peer-reviewed
RseP Peptidase

Yohei Hizukuri, Koreaki Ito, Yoshinori Akiyama

Handbook of Proteolytic Enzymes, 3rd ed., 2 1545-1550, 2013 Peer-reviewed
PDZ domains of RseP are not essential for sequential cleavage of RseA or stress-induced σ(E) activation in vivo

Yohei Hizukuri, Yoshinori Akiyama

Molecular microbiology, 86(5) 1232-1245, Dec, 2012 Peer-reviewed
Post-liberation cleavage of signal peptides is catalyzed by the site-2 protease (S2P) in bacteria

Akira Saito, Yohei Hizukuri, Ei-ichi Matsuo, Shinobu Chiba, Hiroyuki Mori, Osamu Nishimura, Koreaki Ito, Yoshinori Akiyama

Proceedings of the National Academy of Sciences of the United States of America, 108(33) 13740-13745, Aug, 2011 Peer-reviewed
Disulphide cross-linking between the stator and the bearing components in the bacterial flagellar motor

Yohei Hizukuri, Seiji Kojima, Michio Homma

Journal of Biochemistry, 148(3) 309-318, Sep, 2010 Peer-reviewed
The peptidoglycan-binding (PGB) Domain of the Escherichia coli Pal Protein can also Function as the PGB Domain in E. coli Flagellar Motor Protein MotB

Yohei Hizukuri, John Frederick Morton, Toshiharu Yakushi, Seiji Kojima, Michio Hommay

Journal of Biochemistry, 146(2) 219-229, Aug, 2009 Peer-reviewed
Systematic Cys mutagenesis of FIgI, the flagellar P-ring component of Escherichia coli

Yohei Hizukuri, Seiji Kojima, Toshiharu Yakushi, Ikuro Kawagishi, Michio Homma

Microbiology, 154(Pt 3) 810-817, Mar, 2008 Peer-reviewed

Misc.

細菌膜内切断プロテアーゼRsePの基質取り込み・切断時におけるダイナミックな構造変化モデル

三宅拓也, 今泉友希, 高貫一徳, 小林達也, 横山達彦, 大井里香, 檜作洋平, 禾晃和, 秋山芳展

日本生化学会大会プログラム・講演要旨集, 95回 1T07a-07, Nov, 2022
バクテリアの表層変化と生存戦略細菌パーシスター化における膜内切断プロテアーゼRsePの役割生理学的及び構造学的アプローチ

檜作洋平, 横山達彦, 三宅拓也, 小林達也, 今泉友希, 高貫一徳, 大井里香, 禾晃和, 秋山芳展

日本細菌学雑誌, 77(1) 19-19, Feb, 2022
A novel physiological function of RseP, an E. coli intramembrane protease: Transcriptional activation of genes involved in iron uptake through the cleavage of FecR.

横山達彦, 新苗智也, 津曲和哉, 今見考志, 石濱泰, 檜作洋平, 秋山芳展

日本農芸化学会大会講演要旨集(Web), 2021, 2021
TAシステムを介した細菌の休眠と覚醒:生化学と一細胞解析の融和を目指したアプローチ大腸菌膜内切断プロテアーゼRsePを介したTA systemの制御細胞休眠・覚醒における意義

檜作洋平, 横山達彦, 秋山芳展

日本生化学会大会プログラム・講演要旨集, 93回 [3S10m-01], Sep, 2020
バクテリアの表層構造の構築と機能大腸菌内膜で働く膜内切断プロテアーゼRsePの新規切断基質探索パーシスター化制御への関与

檜作洋平, 横山達彦, 秋山芳展

日本細菌学雑誌, 75(1) 15-15, Jan, 2020
大腸菌S2PファミリープロテアーゼRsePの基質探索:鉄取り込みに関与する転写制御因子の切断とその生理的意義

横山達彦, 新苗智也, 津曲和哉, 今見考志, 石濱泰, 檜作洋平, 秋山芳展

日本生化学会大会(Web), 93rd, 2020
バクテリアの表層構造の構築と機能大腸菌内膜で働く膜内切断プロテアーゼRsePの新規切断基質探索パーシスター化制御への関与

檜作洋平, 横山達彦, 秋山芳展

日本細菌学雑誌, 75(1) 15-15, Jan, 2020 Peer-reviewed
FRET蛍光ペプチドを用いた大腸菌S2Pファミリー膜内切断プロテアーゼのリアルタイム切断kinetics計測系の構築

檜作洋平, 秋山芳展

日本生化学会大会プログラム・講演要旨集, 92回 [1P-080], Sep, 2019
大腸菌膜内切断プロテアーゼRsePの基質切断と構造維持に重要な膜表在・両親媒性ヘリックスの解析

三宅拓也, 檜作洋平, 秋山芳展

日本生化学会大会プログラム・講演要旨集, 92回 [1P-082], Sep, 2019
FRET蛍光ペプチドを用いた大腸菌S2Pファミリー膜内切断プロテアーゼのリアルタイム切断kinetics計測系の構築

檜作洋平, 秋山芳展

日本生化学会大会プログラム・講演要旨集, 92回 [1P-080], Sep, 2019 Peer-reviewed
大腸菌膜内切断プロテアーゼRsePの新規基質切断とその生理的意義低分子膜タンパク質HokBの切断を介したパーシスター形成制御

横山達彦, 檜作洋平, 秋山芳展

日本生化学会大会プログラム・講演要旨集, 92回 [1P-081], Sep, 2019 Peer-reviewed
大腸菌膜内切断プロテアーゼRsePの基質切断と構造維持に重要な膜表在・両親媒性ヘリックスの解析

三宅拓也, 檜作洋平, 秋山芳展

日本生化学会大会プログラム・講演要旨集, 92回 [1P-082], Sep, 2019 Peer-reviewed
大腸菌表層ストレス応答:インプットとアウトプットに働くプロテアーゼの機能

秋山芳展, 秋山光市郎, 岩間(舛井)千草, 大門康志, 檜作洋平, 水野慎也, 宮崎亮次, 森博幸, 禾晃和, 塩田拓也 …

Institute for Fermentation, Osaka. Research Communications, (33), 2019
大腸菌膜内切断プロテアーゼRsePの構造維持と機能調節に関わる新規ドメインの解析

三宅拓也, 檜作洋平, 秋山芳展

日本生化学会大会(Web), 91st ROMBUNNO.2T11e‐05(3P‐114) (WEB ONLY)-114)], 2018
細菌べん毛III型分泌装置構成因子の翻訳後多段階プロセシング

檜作洋平, 鈴木健裕, 照島功祐, 堂前直, 秋山芳展

日本生化学会大会(Web), 91st ROMBUNNO.3P‐118 (WEB ONLY)-118], 2018
超高親和性タグを内部に挿入した複数回膜貫通型タンパク質の精製法の検討

高貫一徳, 大井里香, 有森貴夫, 三宅拓也, 檜作洋平, 秋山芳展, 加藤幸成, 高木淳一, 禾晃和

日本生化学会大会(Web), 90th ROMBUNNO.1P‐1390 (WEB ONLY)-1390], 2017
細菌RhomboidファミリープロテアーゼGlpGはべん毛III型分泌装置の構成因子の分解に関与する

檜作洋平, 照島功祐, 秋山芳展

日本生化学会大会(Web), 90th ROMBUNNO.4P1T25‐04(3P‐0153) (WEB ONLY)-0153)], 2017
細菌の表層ストレス応答に関わる膜内切断プロテアーゼRsePの新規機能調節領域の解析

三宅拓也, 檜作洋平, 秋山芳展

日本生化学会大会(Web), 90th ROMBUNNO.2P‐0151 (WEB ONLY)-0151], 2017
細菌RhomboidプロテアーゼGlpGの生理的切断基質の探索及びべん毛III型分泌装置との関係

檜作洋平, 照島功祐, 秋山芳展

日本生化学会大会(Web), 89th ROMBUNNO.3T11‐06(3P‐114) (WEB ONLY), 2016
細菌表層ストレス応答を制御するS2Pファミリー膜内切断プロテアーゼの細胞内イメージング解析

檜作洋平, 秋山芳展

日本生化学会大会(Web), 88th 4T17P-15(3P0372) (WEB ONLY), 2015

Books and Other Publications

膜内切断プロテアーゼの基質認識・切断機構

檜作洋平 (Role: Contributor, 生物物理 vol.59 no.2 pp.079-083)

一般社団法人日本生物物理学会, Apr, 2019
Chapter One - Biochemical Characterization of Function and Structure of RseP, an Escherichia coli S2P Protease. in Methods in Enzymology

Y. Hizukuri, K. Akiyama, Y. Akiyama (Role: Contributor, pp.1-33)

Elsevier Inc., 2017
「膜内部での蛋白質分解とストレス応答制御」別冊・医学のあゆみ「レドックスUPDATE ―ストレス制御の臨床医学・健康医学」（監修：平家俊男，淀井淳司）

檜作洋平, 秋山芳展 (Role: Contributor, pp.69-74)

医歯薬出版株式会社, 2015
RseP peptidase. in Handbook of Proteolytic Enzymes, 3rd ed. (ed. Rawlings, N. D. and Salvesen, G.)

Hizukuri, Y, Ito, K, Akiyama, Y (Role: Contributor, pp.1546-1550)

Elsevier Ltd., Jan, 2013

Presentations

Crystal structure and novel gating mechanistic model to regulate substrate entry and its cleavage of the intramembrane protease RseP

Yohei Hizukuri, Yuki Imaizumi, Kazunori Takanuki, Takuya Miyake, Tatsuya Kobayashi, Tatsuhiko Yokoyama, Rika Oi, Terukazu Nogi, Yoshinori Akiyama

Nov 15, 2023
Structure-based gating model for substrate accommodation and intramembrane proteolysis of E. coli site 2-protease (S2P), RseP

Yohei Hizukuri, Yuki Imaizumi, Kazunori Takanuki, Takuya Miyake, Tatsuya Kobayashi, Tatsuhiko Yokoyama, Rika Oi, Terukazu Nogi, Yoshinori Akiyama

IPS2023, 12th General Meeting of the International Proteolysis Society (Singapore), Jun 28, 2023
膜内切断プロテアーゼRsePのゲーティング機構モデルの検証と基質特異的認識に関する解析

檜作洋平

2022年度国立遺伝学研究所研究会「単細胞生物に見られる生体プロセスの恒常性維持システム」, Mar 30, 2023 Invited
Verification of the substrate recognition and cleavage specificity of RseP, an Escherichia coli S2P family intramembrane protease, by systematic mutational analysis of substrates

Yohei Hizukuri, Daiyu Furumi, Yoshinori Akiyama

Nov 10, 2022
Verification of the substrate recognition and cleavage specificity of RseP, an Escherichia coli S2P family intramembrane protease, by systematic mutational analysis of substrates

Yohei Hizukuri, Daiyu Furumi, Yoshinori Akiyama

Nov 10, 2022
Role of intramembrane protease RseP in bacterial persistence: Physiological/structural approaches

Yohei Hizukuri, Tatsuhiko Yokoyama, Takuya Miyake, Tatsuya Kobayashi, Yuki Imaizumi, Kazunori Takanuki, Rika Oi, Terukazu Nogi, Yoshinori Akiyama

Mar 29, 2022 Invited
立体構造に基づく膜内切断プロテアーゼRsePの基質取り込み・切断制御機構

檜作洋平

2021年度国立遺伝学研究所研究会「単細胞システムの複製と維持における生体高分子の機能」, Mar 8, 2022 Invited
細菌膜内切断プロテアーゼの制御機構と生理機能

檜作洋平

第3回生命理学研究会(名古屋), Mar 13, 2021
Regulation of the TA system mediated by an intramembrane protease RseP in Escherichia coli: a physiological significance in persister formation and awakening of cells

Yohei Hizukuri, Tatsuhiko Yokoyama, Yoshinori Akiyama

Sep 16, 2020 Invited
大腸菌膜内切断プロテアーゼRsePの切断基質探索から明らかとなってきた新奇生理機能

檜作洋平

宮崎大学第1回微生物化学研究室セミナー, Sep 27, 2019 Invited
FRETリアルタイム活性測定系を用いた大腸菌S2Pファミリー膜内切断プロテアーゼRsePのkinetics解析

檜作洋平, 秋山芳展

Sep 25, 2019
Construction of a real-time enzymatic activity assay system for kinetic analysis of a bacterial S2P family intramembrane protease using FRET peptide substrate

Yohei Hizukuri, Yoshinori Akiyama

Sep 19, 2019
Construction of a real-time enzymatic activity assay system for kinetic analysis of a bacterial S2P family intramembrane protease using FRET peptide substrate

Yohei Hizukuri, Yoshinori Akiyama

Sep 18, 2019
膜内切断プロテアーゼRsePの新規切断基質の探索及びkinetics解析の試み

檜作洋平

2018年度国立遺伝学研究所研究会「単細胞生物における細胞装置の機能と連携」, Mar 18, 2019 Invited
膜内切断プロテアーゼは如何にして脂質二重層内部で基質を認識・切断するか？

檜作洋平

京都大学第49回京化異分科交流若手サロン, Oct 25, 2018 Invited
Multistep post-translational processing in the component of the bacterial flagellar type III secretion apparatus

Yohei Hizukuri, Takehiro Suzuki, Kosuke Terushima, Naoshi Dohmae, Yoshinori Akiyama

Sep 26, 2018
A component of the bacterial flagellar type III secretion apparatus receives multistep post-translational processing

檜作洋平, 鈴木健裕, 照島功祐, 堂前直, 秋山芳展

Sep 16, 2018
Multistep post-translational proteolysis/modification of a component of the bacterial flagellar type III secretion apparatus

Yohei Hizukuri, Takehiro Suzuki, Kosuke Terushima, Naoshi Dohmae, Yoshinori Akiyama

Aug 28, 2018
Bacterial Rhomboid family protease GlpG is involved in a degradation of a component of the flagellar type III secretion apparatus

Yohei Hizukuri, Kosuke Terushima, Yoshinori Akiyama

Dec 9, 2017
Bacterial Rhomboid family protease GlpG is involved in a degradation of a component of the flagellar type III secretion apparatus

Yohei Hizukuri, Kosuke Terushima, Yoshinori Akiyama

Dec 8, 2017

Professional Memberships

Research Projects

新規構造・基質に基づく細菌S2P膜内切断プロテアーゼの切断制御機構の酵素学的理解

科学研究費助成事業基盤研究(C), 基盤研究(C), 日本学術振興会, Apr, 2022 - Mar, 2025

檜作洋平
細菌Ⅲ型分泌装置構成因子が受ける新奇な翻訳後多段階プロセシングの分子機構

科学研究費助成事業基盤研究(C), 基盤研究(C), 日本学術振興会, Apr, 2019 - Mar, 2022

檜作洋平
Physiological functions and selective substrate-cleavage mechanism of E. coli intramembrane protease RseP

Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, Apr, 2018 - Mar, 2021

Akiyama Yoshinori
Analysis of regulation mechanism of flagellar type III secretion apparatus mediated by bacterial rhomboid intramembrane protease GlpG

Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B), Grant-in-Aid for Young Scientists (B), Japan Society for the Promotion of Science, Apr, 2017 - Mar, 2020

Hizukuri Yohei
Elucidation of substrate recognition mechanism of I-CLiP in the trans- and juxta-membrane regions

Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, Apr, 2014 - Mar, 2018

NOGI Terukazu, AKIYAMA Yoshinori, HIZUKURI Yohei, OI Rika, TAKANUKI Kazunori, TAMURA Risako
Live-cell imaging of a bacterial extracytoplasmic stress response and exploration of a novel function of an intramembrane protease that regulates the stress response

Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B), Grant-in-Aid for Young Scientists (B), Japan Society for the Promotion of Science, Apr, 2014 - Mar, 2017

HIZUKURI Yohei
膜を超えた情報伝達：細胞表層ストレス応答システムに対する新視点からのアプローチ

科学研究費助成事業特別研究員奨励費(PD), 特別研究員奨励費, 日本学術振興会, 2012 - Mar, 2014

檜作洋平
超分子ナノマシン細菌べん毛モーターを再構成して機能を探る

科学研究費助成事業特別研究員奨励費(DC2), 特別研究員奨励費, 日本学術振興会, 2007 - 2008

檜作洋平

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Misc.

Books and Other Publications

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