論文

査読有り
2018年

A novel role for scavenger receptor B1 as a contributor to the capture of specific volatile odorants in the nasal cavity

Biomedical Research (Japan)
  • Satoshi Tsuzuki
  • ,
  • Yusaku Kimoto
  • ,
  • Shinhye Lee
  • ,
  • Tatsuya Sugawara
  • ,
  • Yuki Manabe
  • ,
  • Kazuo Inoue

39
3
開始ページ
117
終了ページ
129
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.2220/biomedres.39.117
出版者・発行元
Biomedical Research Foundation

Class B scavenger receptors, scavenger receptor B1 (SR-B1) and cluster of differentiation 36 (CD36), are broadly expressed cell-surface proteins and are believed to serve as multifaceted players in lipid and lipoprotein metabolism in mammals. Because of its ability to recognise distinct odour-active volatile compounds and its presence in murine olfactory epithelium, CD36 has recently emerged as a participant in the detection of odorants within the nasal cavity. However, there have been no attempts to assess whether SR-B1 has such a role. In this study, we performed a cell-free in-vitro assay utilising a peptide mimic of the receptor, and demonstrated that SR-B1 could recognise aliphatic aldehydes (e.g., tetradecanal), a distinct class of volatile odorants, as potential ligands. By reverse transcription-polymerase chain reaction and western immunoblot analyses, we detected the expression of SR-B1 mRNA and protein, respectively, in mouse olfactory tissue. Finally, we immunohistochemically mapped the distribution of SR-B1 in the surface layer of olfactory epithelium in vivo, which is the first line of odorant detection. These findings uncover a novel role for SR-B1 as a contributor to the capture of specific odorants in the nasal cavity of mammals.

リンク情報
DOI
https://doi.org/10.2220/biomedres.39.117
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/29899187
Scopus Url
http://www.scopus.com/inward/record.url?eid=2-s2.0-85048594536&partnerID=MN8TOARS
URL
http://orcid.org/0000-0002-5663-0074
ID情報
  • DOI : 10.2220/biomedres.39.117
  • ISSN : 1880-313X
  • ISSN : 0388-6107
  • ORCIDのPut Code : 53187239
  • PubMed ID : 29899187
  • SCOPUS ID : 85048594536

エクスポート
BibTeX RIS