2018年
A novel role for scavenger receptor B1 as a contributor to the capture of specific volatile odorants in the nasal cavity
Biomedical Research (Japan)
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- 巻
- 39
- 号
- 3
- 開始ページ
- 117
- 終了ページ
- 129
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.2220/biomedres.39.117
- 出版者・発行元
- Biomedical Research Foundation
Class B scavenger receptors, scavenger receptor B1 (SR-B1) and cluster of differentiation 36 (CD36), are broadly expressed cell-surface proteins and are believed to serve as multifaceted players in lipid and lipoprotein metabolism in mammals. Because of its ability to recognise distinct odour-active volatile compounds and its presence in murine olfactory epithelium, CD36 has recently emerged as a participant in the detection of odorants within the nasal cavity. However, there have been no attempts to assess whether SR-B1 has such a role. In this study, we performed a cell-free in-vitro assay utilising a peptide mimic of the receptor, and demonstrated that SR-B1 could recognise aliphatic aldehydes (e.g., tetradecanal), a distinct class of volatile odorants, as potential ligands. By reverse transcription-polymerase chain reaction and western immunoblot analyses, we detected the expression of SR-B1 mRNA and protein, respectively, in mouse olfactory tissue. Finally, we immunohistochemically mapped the distribution of SR-B1 in the surface layer of olfactory epithelium in vivo, which is the first line of odorant detection. These findings uncover a novel role for SR-B1 as a contributor to the capture of specific odorants in the nasal cavity of mammals.
- リンク情報
- ID情報
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- DOI : 10.2220/biomedres.39.117
- ISSN : 1880-313X
- ISSN : 0388-6107
- ORCIDのPut Code : 53187239
- PubMed ID : 29899187
- SCOPUS ID : 85048594536