MISC

査読有り 国際誌
2014年

Visualization of Notch signaling oscillation in cells and tissues.

Methods in molecular biology (Clifton, N.J.)
  • Hiromi Shimojo
  • ,
  • Yukiko Harima
  • ,
  • Ryoichiro Kageyama

1187
開始ページ
169
終了ページ
79
記述言語
英語
掲載種別
DOI
10.1007/978-1-4939-1139-4_13

The Notch signaling effectors Hes1 and Hes7 exhibit oscillatory expression with a period of about 2-3 h during embryogenesis. Hes1 oscillation is important for proliferation and differentiation of neural stem cells, whereas Hes7 oscillation regulates periodic formation of somites. Continuous expression of Hes1 and Hes7 inhibits these developmental processes. Thus, expression dynamics are very important for gene functions, but it is difficult to distinguish between oscillatory and persistent expression by conventional methods such as in situ hybridization and immunostaining. Here, we describe time-lapse imaging methods using destabilized luciferase reporters and a highly sensitive cooled charge-coupled device camera, which can monitor dynamic gene expression. Furthermore, the expression of two genes can be examined simultaneously by a dual reporter system using two-color luciferase reporters. Time-lapse imaging analyses reveal how dynamically gene expression changes in many biological events.

リンク情報
DOI
https://doi.org/10.1007/978-1-4939-1139-4_13
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/25053489
URL
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84907334409&origin=inward
ID情報
  • DOI : 10.1007/978-1-4939-1139-4_13
  • ISSN : 1064-3745
  • PubMed ID : 25053489
  • SCOPUS ID : 84907334409

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