The dynamic behaviors of the enzymes and DNA structural changes were visualized using the designed DNA origami structures and high-speed AFM. The movement and the reactions of DNA methyltransferase, DNA repair enzymes, and RNA polymerase were observed by introducing the substrate double-stranded DNAs (dsDNA) to the DNA nanostructures. For the formation and disruption of G-quadruplex and double-stranded DNA, the two dsDNA incorporated in the DNA frame can amplify the small structural change to the global structural change, which enable the visualization of the reactions. The stepwise strand migration of a DNA nanomachine was visualized and analyzed on the DNA track constructed on the DNA origami surface.<br>