2015年
Folding RNA-Protein Complex into Designed Nanostructures.
Methods in molecular biology (Clifton, N.J.)
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- 巻
- 1316
- 号
- 開始ページ
- 169
- 終了ページ
- 79
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1007/978-1-4939-2730-2_14
RNA-protein (RNP) complexes are promising biomaterials for the fields of nanotechnology and synthetic biology. Protein-responsive RNA sequences (RNP motifs) can be integrated into various RNAs, such as messenger RNA, short-hairpin RNA, and synthetic RNA nano-objects for a variety of purposes. Direct observation of RNP interaction in solution at high resolution is important in the design and construction of RNP-mediated nanostructures. Here we describe a method to construct and visualize RNP nanostructures that precisely arrange a target protein on the RNA scaffold with nanometer scale. High-speed AFM (HS-AFM) images of RNP nanostructures show that the folding of RNP complexes of defined sizes can be directly visualized at single RNP resolution in solution.
- リンク情報
- ID情報
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- DOI : 10.1007/978-1-4939-2730-2_14
- ISSN : 1064-3745
- PubMed ID : 25967061