2014年9月
NanoScript: A Nanoparticle-Based Artificial Transcription Factor for Effective Gene Regulation
ACS NANO
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- 巻
- 8
- 号
- 9
- 開始ページ
- 8959
- 終了ページ
- 8967
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1021/nn501589f
- 出版者・発行元
- AMER CHEMICAL SOC
Transcription factor (TF) proteins are master regulators of transcriptional activity and gene expression. TF-based gene regulation is a promising approach for many biological applications; however, several limitations hinder the full potential of TFs. Herein, we developed an artificial, nanoparticle-based transcription factor, termed NanoScript, which is designed to mimic the structure and function of TFs. NanoScript was constructed by tethering functional peptides and small molecules called synthetic transcription factors, which mimic the individual TF domains, onto gold nanoparticles. We demonstrate that NanoScript localizes within the nucleus and initiates transcription of a reporter plasmid by over 15-fold. Moreover, NanoScript can effectively transcribe targeted genes on endogenous DNA in a nonviral manner. Because NanoScript is a functional replica of TF proteins and a tunable gene-regulating platform, it has great potential for various stem cell applications.
- リンク情報
- ID情報
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- DOI : 10.1021/nn501589f
- ISSN : 1936-0851
- eISSN : 1936-086X
- PubMed ID : 25133310
- Web of Science ID : WOS:000342184400024