2014年5月
Single Molecule Visualization and Characterization of Sox2-Pax6 Complex Formation on a Regulatory DNA Element Using a DNA Origami Frame
NANO LETTERS
- ,
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- ,
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- 巻
- 14
- 号
- 5
- 開始ページ
- 2286
- 終了ページ
- 2292
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1021/nl4044949
- 出版者・発行元
- AMER CHEMICAL SOC
We report the use of atomic force microscopy (AFM) to study Sox2-Pax6 complex formation on the regulatory DNA element at a single molecule level. Using an origami DNA scaffold containing two DNA strands with different levels of tensile force, we confirmed that DNA bending is necessary for Sox2 binding. We also demonstrated that two transcription factors bind cooperatively by observing the increased occupancy of Sox2-Pax6 on the DNA element compared to that of Sox2 alone.
- リンク情報
- ID情報
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- DOI : 10.1021/nl4044949
- ISSN : 1530-6984
- eISSN : 1530-6992
- PubMed ID : 24660747
- Web of Science ID : WOS:000336074800008