2001年1月
Mutagenic effects of 5-formyluracil on a plasmid vector during replication in Escherichia coli
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
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- 巻
- 77
- 号
- 1
- 開始ページ
- 53
- 終了ページ
- 58
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1080/095530001453113
- 出版者・発行元
- TAYLOR & FRANCIS LTD
Purpose : 5-Formyluracil (5-foU) is a major derivative of thymine produced in DNA by ionizing radiation and various chemical oxidants. It has been previously shown that 5-foU in template DNA directs misincorporation of nucleotides by DNA polymerases during in vitro DNA synthesis. The present experiments were designed to understand the biological effects of 5-foU in vivo.
Materials and methods: The modified base was incorporated site-specifically into the recognition site of restriction endonuclease SalI (5'-GTCGAC) or AflII (5'-CTTAAG) in vector plasmid pSVK3 and introduced the plasmid into Escherichia coli.
Results: When the plasmids were replicated in E. coli, 5-foU caused mutations at the target sites. The induced mutation frequencies were 0.038-0.049%. Sequence analysis revealed that 5-foU preferentially caused T:A --> C:G and T: A --> A:T base substitutions and -1 deletions at the 5-foU site. 5-FoU also caused mutations at sites near the 5-foU. The alkA mutation did not affect the frequency of mutations in 5-foU-containing plasmids.
Conclusions : The present experiments demonstrated that 5-formyluracil in DNA caused mutations in E. coli.
Materials and methods: The modified base was incorporated site-specifically into the recognition site of restriction endonuclease SalI (5'-GTCGAC) or AflII (5'-CTTAAG) in vector plasmid pSVK3 and introduced the plasmid into Escherichia coli.
Results: When the plasmids were replicated in E. coli, 5-foU caused mutations at the target sites. The induced mutation frequencies were 0.038-0.049%. Sequence analysis revealed that 5-foU preferentially caused T:A --> C:G and T: A --> A:T base substitutions and -1 deletions at the 5-foU site. 5-FoU also caused mutations at sites near the 5-foU. The alkA mutation did not affect the frequency of mutations in 5-foU-containing plasmids.
Conclusions : The present experiments demonstrated that 5-formyluracil in DNA caused mutations in E. coli.
- リンク情報
- ID情報
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- DOI : 10.1080/095530001453113
- ISSN : 0955-3002
- PubMed ID : 11213350
- Web of Science ID : WOS:000166479900006