2008年
Sequence-specific alkylation by Y-shaped and tandem hairpin pyrrole-imidazole polyamides
CHEMISTRY-A EUROPEAN JOURNAL
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- 巻
- 14
- 号
- 3
- 開始ページ
- 864
- 終了ページ
- 870
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1002/chem.200700571
- 出版者・発行元
- WILEY-BLACKWELL
To extend the target DNA sequence length of the hairpin pyrrole-imidazole (Py-Im) polyamide 1, we designed and synthesized Y-shaped and tandem hairpin Py-Im polyamides 2 and 3, which possess 1-(chloromethyl)-5-hydroxy-1,2-dihydro-3H-benz[e]indole (seco-CBI) as DNA-alkylating moieties. High-resolution denaturing polyacrylamide gel electrophoresis by using 5'-Texas-Red-labeled 465 base pair (bp) DNA fragments revealed that conjugates 2 and 3 alkylated the adenine of the target DNA sequences at nanomolar concentrations. Conjugate 2 alkylated adenine N3 at the 3' end of two 8 bp match sequences, 5'-AATAACCA-3' (site A) and 5'-AAATTCCA-3' (site C), while conjugate 3 recognized one 10bp match sequence, 5'-AGAATAACCA-3' (siteA) in the 465 bp DNA fragments. These results demonstrate that seco-CBI conjugates of Y-shaped and tandem hairpin polyamides have extended their target alkylation sequences.
- リンク情報
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- DOI
- https://doi.org/10.1002/chem.200700571
- J-GLOBAL
- https://jglobal.jst.go.jp/detail?JGLOBAL_ID=200902240269570515
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/18000923
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000252643100010&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1002/chem.200700571
- ISSN : 0947-6539
- J-Global ID : 200902240269570515
- PubMed ID : 18000923
- Web of Science ID : WOS:000252643100010