2013年1月23日
Direct and real-time observation of rotary movement of a DNA nanomechanical device
Journal of the American Chemical Society
- ,
- ,
- ,
- 巻
- 135
- 号
- 3
- 開始ページ
- 1117
- 終了ページ
- 1123
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1021/ja310454k
Analogous to the biologically abundant protein-based linear molecular machines that translocate along their target surface, we have recently constructed the DNA-based synthetic molecular motors that effect linear movement or navigate a network of tracks on a DNA origami substrate. However, a DNA-based molecular machine with rotary function, analogous to rotary proteins, is still unexplored. Here, we report the construction of a rotary motor based on the B-Z conformational transition of DNA and the direct and real-time observation of its function within a frame-shaped DNA origami. The motor can be switched off by introducing conditions that stabilize B-DNA, while it can be fueled by adding Z-DNA-promoting high-saline buffer. When MgCl2 was used as external stimulus, 70% of the motors rotated, while 76% of the stators/controls exhibited no rotation. Such a motor system could be successfully applied to perform multiple actions aimed for our benefit. Moreover, for the first time we have directly observed the B-Z conformational transition of DNA in real-time, which shed light on the fundamental understanding of DNA conformations. © 2012 American Chemical Society.
- リンク情報
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- DOI
- https://doi.org/10.1021/ja310454k
- J-GLOBAL
- https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201302255807210877
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/23311576
- Scopus
- https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84872824931&origin=inward
- Scopus Citedby
- https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84872824931&origin=inward
- ID情報
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- DOI : 10.1021/ja310454k
- ISSN : 0002-7863
- ISSN : 1520-5126
- eISSN : 1520-5126
- J-Global ID : 201302255807210877
- PubMed ID : 23311576
- SCOPUS ID : 84872824931