2006年
Oxygen consumption of cell suspension in a poly(dimethylsiloxane) (PDMS) microchannel estimated by scanning electrochemical microscopy
ANALYST
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- 巻
- 131
- 号
- 9
- 開始ページ
- 1006
- 終了ページ
- 1011
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1039/b600080k
- 出版者・発行元
- ROYAL SOC CHEMISTRY
A quantitative analysis of the oxygen concentration profile near a poly(dimethylsiloxane) (PDMS) microfluidic device was performed using scanning electrochemical microscopy (SECM). A microchannel filled with sodium sulfite (Na2SO3) aqueous solution was imaged by SECM, showing that the oxygen diffusion layer of the PDMS microchannel was observed to be hemicylindrical. Based on a theoretical analysis of the hemicylindrical diffusion layer of the microchannel, the total oxygen mass transfer rates of oxygen to the PDMS microchannel filled with the Na2SO3 solution was calculated to be (4.01 +/- 0.30)x10(-12) mol s(-1). This is the maximum value of the oxygen transfer rate for this PDMS microchannel device. The oxygen consumption rate increased almost linearly with the logarithm of the concentration of E. coli cells (10(6)similar to 10(8) cells). The respiratory activity for a single E. coli cell was estimated to be similar to 4.31x10(-20) mol s(-1) cell(-1).
- リンク情報
- ID情報
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- DOI : 10.1039/b600080k
- ISSN : 0003-2654
- Web of Science ID : WOS:000240059500006