論文

査読有り 国際誌
2008年11月15日

Molecular cloning and functional characterization of cynomolgus monkey multidrug resistance-associated protein 2 (MRP2).

European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences
  • Masa Yasunaga
  • ,
  • Masaaki Takemura
  • ,
  • Kyoko Fujita
  • ,
  • Hikaru Yabuuchi
  • ,
  • Morimasa Wada

35
4
開始ページ
326
終了ページ
334
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.ejps.2008.08.001

The monkey is an important experimental model in the pharmacological evaluation of new drugs. We isolated monkey multidrug resistance-associated protein 2 (MRP2) cDNA to examine expression profiles among various tissues and measured ATPase activity to assess substrate specificity. The amino acid sequence encoded by monkey MRP2 cDNA was very similar (96% identity) to the reported human MRP2 cDNA (GenBank accession no. NM_000392). The tissue distribution of MRP2 in monkeys was partially different from that in humans. We found relatively high expression of MRP2 in the monkey kidney and small intestine using Northern blotting. Substrate specificity was compared between human and monkey MRP2. The affinity of 17beta-estradiol 17-(beta-d-glucuronide), methotrexate, vinblastine, and probenecid to monkey MRP2 was higher than that to human MRP2. Functional and expression differences between human and monkey MRP2 should be incorporated into the evaluation of candidate drugs.

リンク情報
DOI
https://doi.org/10.1016/j.ejps.2008.08.001
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/18761402
ID情報
  • DOI : 10.1016/j.ejps.2008.08.001
  • PubMed ID : 18761402

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