論文

筆頭著者 国際誌
2020年12月

Organelle membrane-specific chemical labeling and dynamic imaging in living cells

Nature Chemical Biology
  • Tomonori Tamura
  • ,
  • Alma Fujisawa
  • ,
  • Masaki Tsuchiya
  • ,
  • Yuying Shen
  • ,
  • Kohjiro Nagao
  • ,
  • Shin Kawano
  • ,
  • Yasushi Tamura
  • ,
  • Toshiya Endo
  • ,
  • Masato Umeda
  • ,
  • Itaru Hamachi

16
12
開始ページ
1361
終了ページ
1367
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/s41589-020-00651-z
出版者・発行元
Springer Science and Business Media LLC

Lipids play crucial roles as structural elements, signaling molecules and material transporters in cells. However, the functions and dynamics of lipids within cells remain unclear because of a lack of methods to selectively label lipids in specific organelles and trace their movement by live-cell imaging. We describe here a technology for the selective labeling and fluorescence imaging (microscopic or nanoscopic) of phosphatidylcholine in target organelles. This approach involves the metabolic incorporation of azido-choline, followed by a spatially limited bioorthogonal reaction that enables the visualization and quantitative analysis of interorganelle lipid transport in live cells. More importantly, with live-cell imaging, we obtained direct evidence that the autophagosomal membrane originates from the endoplasmic reticulum. This method is simple and robust and is thus powerful for real-time tracing of interorganelle lipid trafficking.

リンク情報
DOI
https://doi.org/10.1038/s41589-020-00651-z
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/32958953
URL
http://www.nature.com/articles/s41589-020-00651-z.pdf
URL
http://www.nature.com/articles/s41589-020-00651-z
ID情報
  • DOI : 10.1038/s41589-020-00651-z
  • ISSN : 1552-4450
  • eISSN : 1552-4469
  • PubMed ID : 32958953

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