論文

査読有り 国際誌
2019年3月15日

Development of a Nitric Oxide-Responsive Labeling Reagent for Proteome Analysis of Live Cells.

ACS chemical biology
  • Yuki Nishikawa
  • ,
  • Takayuki Miki
  • ,
  • Masashi Awa
  • ,
  • Keiko Kuwata
  • ,
  • Tomonori Tamura
  • ,
  • Itaru Hamachi

14
3
開始ページ
397
終了ページ
404
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1021/acschembio.8b01021
出版者・発行元
AMER CHEMICAL SOC

Nitric oxide (NO) is a pleiotropic signaling molecule involved in the regulation of diverse physiological and pathophysiological mechanisms in cardiovascular, nervous, and immunological systems. To understand the biological functions of NO in detail, comprehensive characterization of proteins found in high-NO concentration environments is crucial. Herein, we describe the design of NO-responsive protein labeling reagents based on N-alkoxyacyl- o-phenylenediamine as an optimal reactive scaffold. The designed molecules can label proteins in murine macrophage cells in response to endogenously produced NO. The combination of NO-responsive protein labeling and liquid chromatography-tandem mass spectrometry technology allowed the characterization of the proteome under NO-generated conditions. Moreover, we demonstrated that our reagent was able to selectively mark and be used to fluorescently visualize NO-producing cells in a mixed cell culture system.

リンク情報
DOI
https://doi.org/10.1021/acschembio.8b01021
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/30715847
ID情報
  • DOI : 10.1021/acschembio.8b01021
  • ISSN : 1554-8929
  • PubMed ID : 30715847

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