論文

査読有り 国際誌
2016年6月22日

A Set of Organelle-Localizable Reactive Molecules for Mitochondrial Chemical Proteomics in Living Cells and Brain Tissues.

Journal of the American Chemical Society
  • Yuki Yasueda
  • ,
  • Tomonori Tamura
  • ,
  • Alma Fujisawa
  • ,
  • Keiko Kuwata
  • ,
  • Shinya Tsukiji
  • ,
  • Shigeki Kiyonaka
  • ,
  • Itaru Hamachi

138
24
開始ページ
7592
終了ページ
602
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1021/jacs.6b02254
出版者・発行元
AMER CHEMICAL SOC

Protein functions are tightly regulated by their subcellular localization in live cells, and quantitative evaluation of dynamically altered proteomes in each organelle should provide valuable information. Here, we describe a novel method for organelle-focused chemical proteomics using spatially limited reactions. In this work, mitochondria-localizable reactive molecules (MRMs) were designed that penetrate biomembranes and spontaneously concentrate in mitochondria, where protein labeling is facilitated by the condensation effect. The combination of this selective labeling and liquid chromatography-mass spectrometry (LC-MS) based proteomics technology facilitated identification of mitochondrial proteomes and the profile of the intrinsic reactivity of amino acids tethered to proteins expressed in live cultured cells, primary neurons and brain slices. Furthermore, quantitative profiling of mitochondrial proteins whose expression levels change significantly during an oxidant-induced apoptotic process was performed by combination of this MRMs-based method with a standard quantitative MS technique (SILAC: stable isotope labeling by amino acids in cell culture). The use of a set of MRMs represents a powerful tool for chemical proteomics to elucidate mitochondria-associated biological events and diseases.

リンク情報
DOI
https://doi.org/10.1021/jacs.6b02254
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/27228550
ID情報
  • DOI : 10.1021/jacs.6b02254
  • ISSN : 0002-7863
  • PubMed ID : 27228550

エクスポート
BibTeX RIS