論文

査読有り
2016年3月

Nucleus-selective Chemical Proteomics Using Hoechst-tagged Reactive Molecules

CHEMISTRY LETTERS
  • Yuki Yasueda
  • ,
  • Tomonori Tamura
  • ,
  • Itaru Hamachi

45
3
開始ページ
265
終了ページ
267
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1246/cl.151083
出版者・発行元
CHEMICAL SOC JAPAN

Characterization of a nuclear proteome is a prerequisite for understanding a variety of cellular functions at the molecular level. Here, we report a novel proteomic method based on nucleus selective chemical protein labeling. In our strategy, the Hoechst nuclear staining dye is conjugated with a reactive group, which is distributed in the nucleus of live cells and selectively reacts with nuclear proteins. Using this method, we successfully identified 58 nuclear proteins, containing typical nuclear proteins Histone H2A or Antigen KI-67.

Web of Science ® 被引用回数 : 13

リンク情報
DOI
https://doi.org/10.1246/cl.151083
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000371556600007&DestApp=WOS_CPL
ID情報
  • DOI : 10.1246/cl.151083
  • ISSN : 0366-7022
  • eISSN : 1348-0715
  • Web of Science ID : WOS:000371556600007

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