Papers

Peer-reviewed
Mar, 2016

Nucleus-selective Chemical Proteomics Using Hoechst-tagged Reactive Molecules

CHEMISTRY LETTERS
  • Yuki Yasueda
  • ,
  • Tomonori Tamura
  • ,
  • Itaru Hamachi

Volume
45
Number
3
First page
265
Last page
267
Language
English
Publishing type
Research paper (scientific journal)
DOI
10.1246/cl.151083
Publisher
CHEMICAL SOC JAPAN

Characterization of a nuclear proteome is a prerequisite for understanding a variety of cellular functions at the molecular level. Here, we report a novel proteomic method based on nucleus selective chemical protein labeling. In our strategy, the Hoechst nuclear staining dye is conjugated with a reactive group, which is distributed in the nucleus of live cells and selectively reacts with nuclear proteins. Using this method, we successfully identified 58 nuclear proteins, containing typical nuclear proteins Histone H2A or Antigen KI-67.

Link information
DOI
https://doi.org/10.1246/cl.151083
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000371556600007&DestApp=WOS_CPL
ID information
  • DOI : 10.1246/cl.151083
  • ISSN : 0366-7022
  • eISSN : 1348-0715
  • Web of Science ID : WOS:000371556600007

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