2015年12月
Biomembrane-embedded Catalysts for Membrane-associated Protein Labeling on Red Blood Cells
CHEMISTRY LETTERS
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- 巻
- 44
- 号
- 12
- 開始ページ
- 1673
- 終了ページ
- 1675
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1246/cl.150797
- 出版者・発行元
- CHEMICAL SOC JAPAN
Membrane proteins play crucial roles in fundamental biological processes. Here, we propose a new chemical strategy to label and characterize membrane proteins on cell surface. In this study, 4-dimethylaminopyridine (DMAP) is embedded and concentrated on plasma membrane surface (biomembrane-embedded DMAP: BeD), allowing to selectively modify membrane proteins with synthetic probes such as a fluorophore and an affinity tag. We applied this strategy to human red blood cells, which demonstrated that membrane proteins such as PAS-1 and Band-3 were selectively labeled.
- リンク情報
- ID情報
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- DOI : 10.1246/cl.150797
- ISSN : 0366-7022
- eISSN : 1348-0715
- Web of Science ID : WOS:000365933300014