2014年3月11日
Isolation of human induced pluripotent stem cell-derived dopaminergic progenitors by cell sorting for successful transplantation.
Stem cell reports
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- 巻
- 2
- 号
- 3
- 開始ページ
- 337
- 終了ページ
- 50
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.stemcr.2014.01.013
- 出版者・発行元
- CELL PRESS
Human induced pluripotent stem cells (iPSCs) can provide a promising source of midbrain dopaminergic (DA) neurons for cell replacement therapy for Parkinson's disease. However, iPSC-derived donor cells inevitably contain tumorigenic or inappropriate cells. Here, we show that human iPSC-derived DA progenitor cells can be efficiently isolated by cell sorting using a floor plate marker, CORIN. We induced DA neurons using scalable culture conditions on human laminin fragment, and the sorted CORIN(+) cells expressed the midbrain DA progenitor markers, FOXA2 and LMX1A. When transplanted into 6-OHDA-lesioned rats, the CORIN(+) cells survived and differentiated into midbrain DA neurons in vivo, resulting in significant improvement of the motor behavior, without tumor formation. In particular, the CORIN(+) cells in a NURR1(+) cell-dominant stage exhibited the best survival and function as DA neurons. Our method is a favorable strategy in terms of scalability, safety, and efficiency and may be advantageous for clinical application.
- リンク情報
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- DOI
- https://doi.org/10.1016/j.stemcr.2014.01.013
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/24672756
- PubMed Central
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3964289
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000336647700009&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1016/j.stemcr.2014.01.013
- ISSN : 2213-6711
- PubMed ID : 24672756
- PubMed Central 記事ID : PMC3964289
- Web of Science ID : WOS:000336647700009