Takumi Takata

Profile Information

Affiliation: 准教授, 放射線生命科学研究部門放射線生化学研究分野, 京都大学複合原子力科学研究所

Degree: 理学博士(京都大学)

Researcher number: 80379007
J-GLOBAL ID: 201401030522702123
researchmap Member ID: 7000010668

Research Interests

Research Areas

Research History

Apr, 2021 - Present

JST創発的研究支援事業
Apr, 2020 - Present

Kyoto University Institute for Integrated Radiation and Nuclear Science, Kyoto University
Apr, 2018 - Mar, 2020

Kyoto University Institute for Integrated Radiation and Nuclear Science
Apr, 2017 - Mar, 2018

Research Reactor Institute, Division of Radiation Life Science, Kyoto University
Sep, 2016 - Mar, 2017

KYoto University
Apr, 2015 - Aug, 2016

Tokyo University of Pharmacy and Life Sciences
Oct, 2013 - Mar, 2015

Research Reactor Institute, Kyoto University
Oct, 2009 - Sep, 2013

Massachusetts Institute of Technology
Apr, 2006 - Sep, 2009

Post doc associate, Oregon Health and Science University,

Education

Apr, 2003 - Mar, 2006

京都大学理学研究科化学専攻博士後期課程
Apr, 2001 - Mar, 2003

京都大学理学研究科化学専攻博士前期課程
Apr, 1997 - Mar, 2001

Department of Applied Chemistry, College of Science and Engineering, Ritsumeikan University

Committee Memberships

Awards

Nov, 2017

International Conference of Lens 2017 Travel Award

Takumi Takata
May, 2017

第29回日本白内障学会学術賞, 日本白内障学会

高田匠
Jun, 2016

第115回日本皮膚科学会優秀ポスター賞, 日本皮膚科学会

高田匠
Dec, 2015

Travel Award, International Conference of Lens 2015

Takumi Takata
Aug, 2015

奨励賞, D-アミノ酸学会

高田匠
Jan, 2014

第40回水晶体研究会セッション優秀賞, 水晶体研究会

高田匠

Papers

Eye lens β-crystallins are predicted by native ion mobility-mass spectrometry and computations to form compact higher-ordered heterooligomers.

Amber D Rolland, Takumi Takata, Micah T Donor, Kirsten J Lampi, James S Prell

Structure (London, England : 1993), Jul 11, 2023
Characterization of K-binding factor involved in water-soluble complex of menaquinone-7 produced by Bacillus subtilis natto.

Toshiyuki Chatake, Yasuhide Yanagisawa, Risa Murakami, Tadanori Ohsugi, Hiroyuki Sumi, Takumi Takata, Aya Okuda, Ken Morishima, Rintaro Inoue, Masaaki Sugiyama

Journal of biochemistry, 174(4) 383-389, Jul 7, 2023
Identification of D-amino acid residues in proteins using mass spectrometry

BUNSEKI KAGAKU, 71(6) 319-324, Jun, 2022 Peer-reviewedInvitedLead authorCorresponding author
Elucidation of the mechanism of subunit exchange in αB crystallin oligomers.

Rintaro Inoue, Yusuke Sakamaki, Takumi Takata, Kathleen Wood, Ken Morishima, Nobuhiro Sato, Aya Okuda, Masahiro Shimizu, Reiko Urade, Noriko Fujii …

Scientific reports, 11(1) 2555-2555, Jan 28, 2021
Isomerization of Asp is essential for assembly of amyloid-like fibrils of αA-crystallin-derived peptide.

Kosuke Magami, Naomi Hachiya, Kazuo Morikawa, Noriko Fujii, Takumi Takata

PloS one, 16(4) e0250277, 2021
Effect of a Lens Protein in Low-Temperature Culture of Novel Immortalized Human Lens Epithelial Cells (iHLEC-NY2).

Naoki Yamamoto, Shun Takeda, Natsuko Hatsusaka, Noriko Hiramatsu, Noriaki Nagai, Saori Deguchi, Yosuke Nakazawa, Takumi Takata, Sachiko Kodera, Akimasa Hirata …

Cells, 9(12), Dec 11, 2020
Simultaneous and Rapid Detection of Multiple Epimers and Isomers of Aspartyl Residues in Lens Proteins Using an LC-MS-MRM Method.

Noriko Fujii, Takumi Takata, Ingu Kim, Toshiya Matsubara

ACS omega, 5(42) 27626-27632, Oct 27, 2020
Asp isomerization increases aggregation of α-crystallin and decreases its chaperone activity in human lens of various ages.

Noriko Fujii, Takumi Takata, Ingu Kim, Ken Morishima, Rintaro Inoue, Kousuke Magami, Toshiya Matsubara, Masaaki Sugiyama, Tamaki Koide

Biochimica et biophysica acta. Proteins and proteomics, 1868(9) 140446-140446, Sep, 2020 Peer-reviewed
Site-Specific Rapid Deamidation and Isomerization in Human Lens αA-crystallin in vitro.

Takata T, Ha S, Koide T, Fujii N

Protein Science, 29 955-965, Apr, 2020 Peer-reviewed
Negative charge at aspartate 151 is important for human lens αA-crystallin stability and chaperone function.

Takata T, Matsubara T, Nakamura-Hirota T, Fujii N

Experimental eye research, 182 10-18, May, 2019 Peer-reviewed
D-Amino acids in protein: The mirror of life as a molecular index of aging

Noriko Fujii, Takumi Takata, Norihiko Fujii, Kenzo Aki, Hiroaki Sakaue

Biochimica et Biophysica Acta - Proteins and Proteomics, 1866(7) 840-847, Jul 1, 2018 Peer-reviewedInvited
Identification of Isomeric Aspartate residues in βB2-crystallin from Aged Human Lens

Takumi Takata, Kento Murakami, Atsuhiko Toyama, Noriko Fujii

Biochimica et Biophysica Acta - Proteins and Proteomics, 1866(7) 767-774, Jul 1, 2018 Peer-reviewedInvited
Asp 58 modulates lens αA-crystallin oligomer formation and chaperone function.

Takata T, Nakamura-Hirota T, Inoue R, Morishima K, Sato N, Sugiyama M, Fujii N

The FEBS journal, 285 2263-2277, Apr, 2018 Peer-reviewed
Identification of D-amino acid-containing peptides in human serum

Seongmin Ha, Ingu Kim, Takumi Takata, Tadatoshi Kinouchi, Masaharu Isoyama, Minoru Suzuki, Noriko Fujii

PLOS ONE, 12(12) e0189972, Dec, 2017 Peer-reviewed
Isomeric Replacement of a Single Aspartic Acid Induces a Marked Change in Protein Function: The Example of Ribonuclease A

Hiroaki Sakaue, Tadatoshi Kinouchi, Noriko Fujii, Norihiko Fujii, Takumi Takata

ACS Omega, 2(1) 260-267, Jan 31, 2017 Peer-reviewed
The age related increase of D-amino acids and advanced glycation end-products in protein.

Noriko Fujii, Takumi Takata

4 203-211, 2017 Invited
Ferulic Acid Suppresses Amyloid beta Production in the Human Lens Epithelial Cell Stimulated with Hydrogen Peroxide

Noriaki Nagai, Sachiyo Kotani, Yu Mano, Akina Ueno, Yoshimasa Ito, Toshio Kitaba, Takumi Takata, Noriko Fujii

BIOMED RESEARCH INTERNATIONAL, 2017 5343010, 2017 Peer-reviewed
New insight into the dynamical system of alpha B-crystallin oligomers

Rintaro Inoue, Takumi Takata, Norihiko Fujii, Kentaro Ishii, Susumu Uchiyama, Nobuhiro Sato, Yojiro Oba, Kathleen Wood, Koichi Kato, Noriko Fujii …

SCIENTIFIC REPORTS, 6 29208, Jul, 2016 Peer-reviewed
Aggregation of Trp > Glu point mutants of human gamma-D crystallin provides a model for hereditary or UV-induced cataract

Eugene Serebryany, Takumi Takata, Erika Erickson, Nathaniel Schafheimer, Yongting Wang, Jonathan A. King

PROTEIN SCIENCE, 25(6) 1115-1128, Jun, 2016 Peer-reviewed
Isomerization of Asp residues plays an important role in A-crystallin dissociation

Takumi Takata, Noriko Fujii

FEBS JOURNAL, 283(5) 850-859, Mar, 2016 Peer-reviewed

Misc.

The spontaneous isomerization of aspartate residues in lens crystallin

Takumi Takat, Noriko Fujii

91(3) 322-328, Jun, 2019 Invited
Heat stability and chaperone like function of aged lens protein mimics

Takumi Takata

2019 Invited
蛋白質中のD-アミノ酸と老化

藤井紀子, 高田匠, 金仁求

臨床免疫・アレルギー科, 70 467-474, 2018 Invited
水晶体構成蛋白質中におけるアミノ酸の自発的化学修飾が引き起こす白内障発症機構に関する研究

Takumi Takata

日本白内障学会誌, 30 7-12, 2018 Invited
生命科学におけるパラキラリティーの重要性

Noriko Fujii, Takumi Takata

日本眼薬理, 31 20-27, 2017 Invited
白内障と酸化ストレス

Takumi Takata, Ingu KIm, Noriko Fujii

OCULISTA, 51(51) 28-34, 2017 Invited
D-アミノ酸と老化; 新規老化マーカーとしてのD-アミノ酸の有用性

Noriko Fujii, Takumi Takata

アンチエイジング医学-日本抗加齢学会雑誌, 13(3) 383-391, 2017 Invited
Analysis of the Post-translational Modifications of the Individual Amino Acids in Lens Proteins which were induced by aging and irradiation

52(1) 24-34, 2017 Invited
Age-related Asp Isomerizations in Dissociated Alpha-crystallin from Aged Lens

Takumi Takata, Takashi Sato, Noriko Fujii

INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 57(12), Sep, 2016
A New Rapid Analysis of Amino Acid Isomers in Crystallins of Cataract Lenses: Toward Prevention and Cure of Cataract

Fujii Noriko, Takata Takumi, Fujii Norihiko, Kim Ingu

The Journal of The Japanese Society for Cataract Research, 28(1) 59-62, 2016 Invited
加齢によるタンパク質ホモキラリティーの破れ

Noriko Fujii, Takumi Takata, Hiroaki Sakaue, Norihiko Fujii

Viva Origino, 44(3) 2, 2016 Invited
KINETICS OF SUBUNITS IN α-CRYSTALLIN AS STUDIED BY SMALL-ANGLE NEUTRON SCATTERING

R. Inoue, T. Takata, N. Fujii, N. Sato, Y. Oba, S. Takata, N. Fujii, M. Sugiyama

16th International Conference on Small-Angle Scattering, Sep, 2015
1 Oxidation of amino acid residues of γ-crystallin induced by γ-irradiation(General Session,The 39^<th> Annual Meeting of the SSOEL-Japan Program)

Kim Ingu, Takata Takumi, Saito Takeshi, Fujii Norihiko, Kanamoto Takashi, Fujii Noriko

Viva origino, 42 1-1, 2014
Views and Mini Reviews regarding the 31th annual meeting of the SSOEL-JAPAN

Takata Takumi

Viva origino, 34(3) 118-120, 2006
28 The Research About D-β-Asp Containing Protein in Lens(General Contribution,The 31th annual meeting of the SSOEL-JAPAN (Abstracts))

Takata Takumi, Shimo-Oka Tadashi, Kinouchi Tadatoshi, Fujii Noriko

Viva origino, 34(1) 35-35, 2006
CHARACTERIZATION OF NEW D-BETA-ASPARTATE CONTAINING PROTEINS IN A LENS-DERIVED CELL LINE(Chiral Homogeneity and D-Amino Acids, International Symposium on Origins of Life and Astrobiology) :

Takata Takumi, Shimo-Oka Tadashi, Miki Kunio, Fujii Noriko

Viva origino, 33(2) 126-126, 2005
Joinning in the ISOLAB'05 in Niigata

Takata Takumi

Viva origino, 33(2) 187-187, 2005
Characterization of D-β-Asp containing protein in N/N1003A ce 11 line

TAKATA Takumi, FUJII Noriko, TANAKA Yoshiko, SHIMOOKA Masashi

31(1) 28-28, Mar 30, 2003
EXPRESSION OF D-BETA-ASPARTATE CONTAINING PROTEIN IN LENS-DERlVED CELL LINE

TAKATA Takumi

30(1) 39-39, Mar 1, 2002

Books and Other Publications

The Importance of the Idea of“Parachirality” in Life Science.

Noriko Fujii, Norihiko Fujii, Takumi Takata, Hiroaki Sakaue

Elsevier Inc, 2017 (ISBN: 9780128128244)
D-Amino acids: Physiology, Metabolism, and Application

Noriko Fujii, Takumi Takata, Norihiko Fujii, Kenzo Aki, Hiroaki Sakaue

Springer, 2016 (ISBN: 9784431560753)
皮膚蛋白質中のアスパラギン酸残基異性体と紫外線による皮膚老化の関連性

藤井紀子, 高田匠

シーエムシー出版, 2015
D-Amino Acids: A New Frontier in Amino Acid and Protein Rsearch-practical Methods and Protocol's

Fujii N, Takata T

Nova Science Publishers, New York, 2007

Presentations

134

Analysis of the Susceptible Tryptophan Residues on the Greek Key Motifs of βB2-Crystallin

Jiayue Sun, Takumi Takata

9th International Conference on the Lens, Dec 12, 2023
The site-specific impact of modifications of aspartyl residues on lens αB-crystallin

Daisuke Sasaki, Takumi Takata

9th International Conference on the Lens, Dec 11, 2023
加齢性または先天性白内障モデルβB２-クリスタリン変異体Foldingにおける温度依存性

髙田匠

第62回日本白内障学会総会／第49回水晶体研究会, Jul 22, 2023 Invited
βB2-クリスタリン中のGreek key motif内部トリプトファン残基の重要性

孫嘉玥, 加賀澤悠太, 髙田匠

第62回日本白内障学会・第49回水晶体研究会, Jul 21, 2023
Rapid deamidation/isomerization of Asparagine residues and effect of C-terminal adjacent amino acid residues in human lens αA-crystallin

Takumi Takata

XXVth biennial meeting of the International Society of Eye Research, Feb 22, 2023 Invited
加齢に応じて進行する蛋白質中アミノ酸残基異性化に関する分離分析手法の現状

高田匠

第 50 回構造活性相関シンポジウム, Nov 10, 2022 Invited
水晶体構成タンパク質中アミノ酸残基の傷と白内障

高田匠

第61回日本白内障学会・第48回水晶体研究会, Aug 28, 2022 Invited
βB2-クリスタリン中151番目のトリプトファン残基の重要性

加賀澤悠太, 高田匠

第61回日本白内障学会・第48回水晶体研究会, Aug 28, 2022
Effect of C-terminal adjacent amino acid residues on deamidation/isomerization of asparagine residues in lens component proteins

Takumi Takata

Fifth International Conference on D-Amino Acid Research, Jul 27, 2022 Invited
βB1-クリスタリン中のアスパラギン酸残基の異性化

加賀澤悠太, 金仁求, 高田匠

第60回日本白内障学会総会／第47回水晶体研究会, Nov 26, 2021
水晶体構成蛋白質中アスパラギン酸残基異性化に際するC末端側隣接アミノ酸残基の影響

菅河晴菜, 髙田匠

第60回日本白内障学会総会／第47回水晶体研究会, Nov 26, 2021
Asp残基異性化に応じたα-クリスタリン由来ペプチドの機能及び構造の変化

高田匠, 眞上晃輔

第60回日本白内障学会総会・第47回水晶体研究会合同大会, Nov 26, 2021
Oligomer structure and formation kinetics of cataract-associated proteins revealed with native ion mobility-mass spectrometry

Amber D Rolland, Takumi Takata, Micah T. Donor, Samuel G. Wheeler, Larry L. David, Kirsten J. Lampi, James S. Prell

69th American Society for Mass Spectrometry, Nov 1, 2021
たんぱく質の中にある年輪—新しい老化の指標について—

高田匠

アトムサイエンスフェア講演会2021, Oct 24, 2021 Invited
Creation and evaluation of model proteins in which the isomerization proceeds rapidly in vitro

Sep 11, 2021
Subunit dynamics in alpha-crystallin through deuteration-assisted small-angle neutron scattering

Inoue, Rintaro, Sakamaki, Yusuke, Takata, Takumi, Morishima, Ken, Wood, Kathleen, Sato, Nobuhiro, Okuda, Aya, Shimizu, Masahiro, Urade, Reiko, Fujii, Noriko, Sugiyama, Masaaki

XXV General Assembly and Congress of the International Union of Crystallography - IUCr 2021, Aug 16, 2021
The Effects of Isomerization and Racemization of Asp Residues for Polypeptide Properties

T. Takata, N. Hachiya, N. Fujii

Dec, 2020 Invited
蛋白質結合型のアスパラギン酸残基の異性化修飾について

高田匠, 藤井紀子

第71回日本電気泳動学会総会, Nov, 2020 Invited
クリスタリンの凝集とアスパラギン酸残基の異性化

藤井紀子, 高田匠, 金仁求, 松原稔哉

第59回日本白内障学会総会・第46回水晶体研究会合同大会, May, 2020
αA-クリスタリン中のAsp151をAsnに置換した組換え体では、同部位の異性化が10倍速く進行する

高田匠, 藤井紀子

第59回日本白内障学会総会・第46回水晶体研究会合同学会, May, 2020

Professional Memberships

Research Projects

蛋白質分子内の老化を可視化する革新的研究

科学研究費助成事業基盤研究(C), 基盤研究(C), 日本学術振興会, Apr, 2020 - Mar, 2023

高田匠
蛋白質中D-アミノ酸を基盤とした未知生命科学研究領域の開拓

創発的研究支援事業, 国立研究開発法人科学技術振興機構, Apr, 2021
Isomerization of Aspartate residue as a new indicator of aging

Grants-in-Aid for Scientific Research, Grant-in-Aid for Young Scientists (B), Japan Society for the Promotion of Science, Apr, 2017 - Mar, 2020

Takata Takumi
加齢に応じてタンパク質中に蓄積する異性化アミノ酸残基の生理的意義を解明する研究

科学研究費助成事業国際共同研究加速基金(国際共同研究強化(A)), 国際共同研究加速基金(国際共同研究強化(A)), 日本学術振興会, 2018 - 2018

高田匠
Age-related Asp Isomerizations in Lens Alpha-crystallin

Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B), Grant-in-Aid for Young Scientists (B), Japan Society for the Promotion of Science, Apr, 2015 - Mar, 2017

Takata Takumi

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