Hissing behavior of honey bees is simultaneous wing movement of multiple honey bees, and loud sounds or vibrations are produced from their movements. In previous the study, hissing sound of honey bees has historically been considered as an aposematic signal. This is because hissing is generally observed when disturbance occur, such as mechanical disturbance or visually disturbing stimuli like hornets approaching the colony. Few studies, however, have investigated situations in which honey bees hiss. In this study, 24-h sound recording and video monitoring were conducted to investigate when the Japanese honey bees (Apis cerana japonica) hissed and checked the external environment of the colony when they hissed. As a result, we found that A. cerana japonica hissed not only when hornets approached but also when there was no obvious threatening stimulus outside the colony. In addition, clear diurnal patterns of hissing were observed. It is still not clear whether hissing in A. cerana japonica is more than aposematic signal or not, but our findings of unique occurrence patterns of their hissing raise question about the potential function of hissing behavior in A. cerana japonica.