2015年7月
A highly selective biosynthetic pathway to non-natural C-50 carotenoids assembled from moderately selective enzymes
NATURE COMMUNICATIONS
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- 巻
- 6
- 号
- 開始ページ
- 7534
- 終了ページ
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1038/ncomms8534
- 出版者・発行元
- NATURE PUBLISHING GROUP
Synthetic biology aspires to construct natural and non-natural pathways to useful compounds. However, pathways that rely on multiple promiscuous enzymes may branch, which might preclude selective production of the target compound. Here, we describe the assembly of a six-enzyme pathway in Escherichia coli for the synthesis of C-50-astaxanthin, a non-natural purple carotenoid. We show that by judicious matching of engineered size-selectivity variants of the first two enzymes in the pathway, farnesyl diphosphate synthase (FDS) and carotenoid synthase (CrtM), branching and the production of non-target compounds can be suppressed, enriching the proportion of C-50 backbones produced. We then further extend the C-50 pathway using evolved or wild-type downstream enzymes. Despite not containing any substrate-or product-specific enzymes, the resulting pathway detectably produces only C-50 carotenoids, including similar to 90% C-50-astaxanthin. Using this approach, highly selective pathways can be engineered without developing absolutely specific enzymes.
- リンク情報
- ID情報
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- DOI : 10.1038/ncomms8534
- ISSN : 2041-1723
- Web of Science ID : WOS:000358852300001