論文

査読有り 国際誌
2018年8月

Lactosylceramide synthases encoded by B4galt5 and 6 genes are pivotal for neuronal generation and myelin formation in mice.

PLoS genetics
  • Toru Yoshihara
  • ,
  • Hiroyuki Satake
  • ,
  • Toshikazu Nishie
  • ,
  • Nozomu Okino
  • ,
  • Toshihisa Hatta
  • ,
  • Hiroki Otani
  • ,
  • Chie Naruse
  • ,
  • Hiroshi Suzuki
  • ,
  • Kazushi Sugihara
  • ,
  • Eikichi Kamimura
  • ,
  • Noriyo Tokuda
  • ,
  • Keiko Furukawa
  • ,
  • Koichi Fururkawa
  • ,
  • Makoto Ito
  • ,
  • Masahide Asano

14
8
開始ページ
e1007545
終了ページ
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1371/journal.pgen.1007545

It is uncertain which β4-galactosyltransferase (β4GalT; gene name, B4galt), β4GalT-5 and/or β4GalT-6, is responsible for the production of lactosylceramide (LacCer) synthase, which functions in the initial step of ganglioside biosynthesis. Here, we generated conditional B4galt5 knockout (B4galt5 cKO) mice, using Nestin-Cre mice, and crossed these with B4galt6 KO mice to generate B4galt5 and 6 double KO (DKO) mice in the central nervous system (CNS). LacCer synthase activity and major brain gangliosides were completely absent in brain homogenates from the DKO mice, although LacCer synthase activity was about half its normal level in B4galt5 cKO mice and B4galt6 KO mice. The DKO mice were born normally but they showed growth retardation and motor deficits at 2 weeks and died by 4 weeks of age. Histological analyses showed that myelin-associated proteins were rarely found localized in axons in the cerebral cortex, and axonal and myelin formation were remarkably impaired in the spinal cords of the DKO mice. Neuronal cells, differentiated from neurospheres that were prepared from the DKO mice, showed impairments in neurite outgrowth and branch formation, which can be explained by the fact that neurospheres from DKO mice could weakly interact with laminin due to lack of gangliosides, such as GM1a. Furthermore, the neurons were immature and perineuronal nets (PNNs) were poorly formed in DKO cerebral cortices. Our results indicate that LacCer synthase is encoded by B4galt5 and 6 genes in the CNS, and that gangliosides are indispensable for neuronal maturation, PNN formation, and axonal and myelin formation.

Web of Science ® 被引用回数 : 18

リンク情報
DOI
https://doi.org/10.1371/journal.pgen.1007545
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/30114188
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6095488
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000443389100016&DestApp=WOS_CPL
ID情報
  • DOI : 10.1371/journal.pgen.1007545
  • ISSN : 1553-7404
  • PubMed ID : 30114188
  • PubMed Central 記事ID : PMC6095488
  • Web of Science ID : WOS:000443389100016

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