In this paper, we assumed that the nonverbal communication by using 3DAgents with natural gestures had various advantages compared with only the traditional voice and video communication, and we developed the IMoTS (Interactive Motion Tracking System) to verify this hypothesis. The features of this system are that the natural gestures of 3DAgents are captured easily by using interactive GUI from the 2D video images in which some characteristic human behaviors are captured, transmitted, and reproduced by natural gestures of 3DAgents. From the experimental results, we showed that the accuracy of captured gestures which often used in web communications was within the level of detectable limit. And we found that human behaviors could be characterized by the mathematical formula, and some of the information could be transmitted, especially some personalities such as quirks and likeness had the predominant effects of impressions and memories of human.