Yasukazu Daigaku

Profile Information

Affiliation: Project leader, Cancer Genome Dynamics Project, Cancer Institute, Japanese Foundation for Cancer Research

Degree: Ph.D(Mar, 2006, Tohoku University)

Researcher number: 80619875
J-GLOBAL ID: 201501005080863029
researchmap Member ID: 7000012656

External link: https://www.jfcr.or.jp/english/laboratory/researcher/9530.html

Research Interests

Research Areas

Life sciences / Genomics /

Research History

Nov, 2022 - Present

Project leader, Cancer Genome Dynamics Project, Cancer Institute, Japanese Foundation for Cancer Research
Oct, 2018 - Mar, 2022

PRESTO Researcher (Genome Programming), Japan Science and Technology Agency (JST)
Apr, 2015 - Mar, 2022

Assistant Professor, Frontier Research Institute for Interdisciplinary Sciences, Tohoku University
Jul, 2009 - Mar, 2015

Postdoctoral Research Fellow, Antony Carr lab, Genome Damage and Stability Centre, University of Sussex, UK
Jul, 2011 - Jun, 2013

Oversea Reseach Fellowship, Japan Society for the Promotion of Science (JSPS)
Apr, 2006 - Jun, 2009

Postdoctoral Research Fellow, Helle Ulrich lab, Cancer Research UK London Reseach Insititute

Committee Memberships

Apr, 2019 - Present

評議員, 日本遺伝学会

Awards

Apr, 2020

Distinguished researcher, Tohoku University
Jul, 2018

日本遺伝学会奨励賞

大学保一
Apr, 2017

科学技術分野の文部科学大臣表彰若手科学者賞, 文部科学省

大学保一
Dec, 2015

日本遺伝学会第87回大会 Best Papers (BP) 賞, 日本遺伝学会
Oct, 2015

第23回DNA複製・組換え・修復ワークショップ若手優秀発表賞・口頭部門, 第23回DNA複製・組換え・修復ワークショップ

Papers

Cryo-EM structures of human SPCA1a reveal the mechanism of Ca ²⁺ /Mn ²⁺ transport into the Golgi apparatus

Zhenghao Chen, Satoshi Watanabe, Hironori Hashida, Michio Inoue, Yasukazu Daigaku, Masahide Kikkawa, Kenji Inaba

Science Advances, 9(9), Mar 3, 2023 Peer-reviewed
Novel compound heterozygous mutations in UHRF1 are associated with atypical immunodeficiency, centromeric instability and facial anomalies syndrome with distinctive genome-wide DNA hypomethylation

Motoko Unoki, Guillaume Velasco, Satomi Kori, Kyohei Arita, Yasukazu Daigaku, Wan Kin Au Yeung, Akihiro Fujimoto, Hirofumi Ohashi, Takeo Kubota, Kunio Miyake …

Human Molecular Genetics, 32(9) 1439-1456, Dec 2, 2022 Peer-reviewed
Global landscape of replicative DNA polymerase usage in the human genome

Eri Koyanagi, Yoko Kakimoto, Tamiko Minamisawa, Fumiya Yoshifuji, Toyoaki Natsume, Atsushi Higashitani, Tomoo Ogi, Antony M. Carr, Masato T. Kanemaki, Yasukazu Daigaku

Nature Communications, 13(1) 7221-7221, Nov 24, 2022 Peer-reviewedLast authorCorresponding author
Ubiquitination of DNA Damage-Stalled RNAPII Promotes Transcription-Coupled Repair.

Yuka Nakazawa, Yuichiro Hara, Yasuyoshi Oka, Okiru Komine, Diana van den Heuvel, Chaowan Guo, Yasukazu Daigaku, Mayu Isono, Yuxi He, Mayuko Shimada …

Cell, 180(6) 1228-1244, Mar 19, 2020 Peer-reviewed
Vital roles of PCNA K165 modification during C. elegans gametogenesis and embryogenesis.

Z. Shao, S. Niwa, A. Higashitani, Y. Daigaku

DNA repair, 82 102688, Aug, 2019 Peer-reviewedLast authorCorresponding author
Spatial separation between replisome- and template-induced replication stress signaling

Néstor García-Rodríguez, Magdalena Morawska, Ronald P. Wong, Yasukazu Daigaku, Helle D. Ulrich

EMBO Journal, 37(9) e98369, May 2, 2018 Peer-reviewed
PCNA ubiquitylation ensures timely completion of unperturbed DNA replication in fission yeast

Yasukazu Daigaku, Thomas J. Etheridge, Yuka Nakazawa, Mayumi Nakayama, Adam T. Watson, Izumi Miyabe, Tomoo Ogi, Mark A. Osborne, Antony M. Carr

PLoS Genetics, 13(5) e1006789, May 1, 2017 Peer-reviewedLead authorCorresponding author
Mapping ribonucleotides in genomic DNA and exploring replication dynamics by polymerase usage sequencing (Pu-seq)

Andrea Keszthelyi, Yasukazu Daigaku, Katie Ptasinska, Izumi Miyabe, Antony M. Carr

NATURE PROTOCOLS, 10(11) 1786-1801, Nov, 2015 Peer-reviewed
Polymerase delta replicates both strands after homologous recombination-dependent fork restart

Izumi Miyabe, Ken'Ichi Mizuno, Andrea Keszthelyi, Yasukazu Daigaku, Meliti Skouteri, Saed Mohebi, Thomas A. Kunkel, Johanne M. Murray, Antony M. Carr

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 22(11) 932-938, Nov, 2015 Peer-reviewed
A global profile of replicative polymerase usage

Yasukazu Daigaku, Andrea Keszthelyi, Carolin A. Mueller, Izumi Miyabe, Tony Brooks, Renata Retkute, Mike Hubank, Conrad A. Nieduszynski, Antony M. Carr

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 22(3) 192-198, Mar, 2015 Peer-reviewedLead author
Quantification of DNA-associated proteins inside eukaryotic cells using single-molecule localization microscopy

Thomas J. Etheridge, Remi L. Boulineau, Alex Herbert, Adam T. Watson, Yasukazu Daigaku, Jem Tucker, Sophie George, Peter Joensson, Matthieu Palayret, David Lando …

NUCLEIC ACIDS RESEARCH, 42(19) e146, Oct, 2014 Peer-reviewed
Optimisation of the Schizosaccharomyces pombe urg1 Expression System

Adam T. Watson, Yasukazu Daigaku, Saed Mohebi, Thomas J. Etheridge, Charly Chahwan, Johanne M. Murray, Antony M. Carr

PLOS ONE, 8(12) e83800, Dec, 2013 Peer-reviewed
Action Spectrum Analysis of UVR Genotoxicity for Skin: The Border Wavelengths between UVA and UVB Can Bring Serious Mutation Loads to Skin

Hironobu Ikehata, Shoichi Higashi, Shingo Nakamura, Yasukazu Daigaku, Yoshiya Furusawa, Yasuhiro Kamei, Masakatsu Watanabe, Kazuo Yamamoto, Kotaro Hieda, Nobuo Munakata …

JOURNAL OF INVESTIGATIVE DERMATOLOGY, 133(7) 1850-1856, Jul, 2013 Peer-reviewed
Ubiquitin-dependent DNA damage bypass is separable from genome replication

Yasukazu Daigaku, Adelina A. Davies, Helle D. Ulrich

NATURE, 465(7300) 951-U13, Jun, 2010 Peer-reviewedLead author
Activation of ubiquitin-dependent DNA damage bypass is mediated by replication protein A

Adelina A. Davies, Diana Huttner, Yasukazu Daigaku, Shuhua Chen, Helle D. Ulrich

MOLECULAR CELL, 29(5) 625-636, Mar, 2008 Peer-reviewed
Ames test-negative carcinogen, ortho-phenyl phenol, binds tubulin and causes aneuploidy in budding yeast

Tatsuo Nunoshiba, Eri Watanabe, Teruhisa Takahashi, Yasukazu Daigaku, Satoko Ishikawa, Masataka Mochizuki, Ayako Ui, Takemi Enomoto, Kazuo Yamamoto

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 617(1-2) 90-97, Apr, 2007 Peer-reviewed
Error-free RAD52 pathway and error-prone REVS pathway determines spontaneous mutagenesis in Saccharomyces cereaisiae

Kingo Endo, Yu-ichiro Tago, Yasukazu Daigaku, Kazuo Yamamoto

GENES & GENETIC SYSTEMS, 82(1) 35-42, Feb, 2007 Peer-reviewed
Loss of heterozygosity in yeast can occur by ultraviolet irradiation during the S phase of the cell cycle

Yasukazu Daigaku, Satsuki Mashiko, Keiichiro Mishiba, Saburo Yamamura, Ayako Ui, Takemi Enomoto, Kazuo Yamamoto

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 600(1-2) 177-183, Aug, 2006 Peer-reviewedLead author
Spontaneous mutagenesis in haploid and diploid Saccharomyces cerevisiae

G Ohnishi, K Endo, A Doi, A Fujita, Y Daigaku, T Nunoshiba, K Yamamoto

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 325(3) 928-933, Dec, 2004 Peer-reviewed
Loss of heterozygosity and DNA damage repair in Saccharomyces cerevisiae

Y Daigaku, K Endo, E Watanabe, T Ono, K Yamamoto

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 556(1-2) 183-191, Nov, 2004 Peer-reviewedLead author

Misc.

分裂酵母における複製ＤＮＡポリメラーゼの機能分担のゲノム科学的解析

大学保一

若手研究者が語る２１世紀の遺伝学, (12) P9-P9, Feb 15, 2016
Roadworks of DNA Damage Bypass during and after Replication

Daigaku Y

Genes and Enviroment, 34(2) 77-88, May 19, 2012

Books and Other Publications

Genome Instability. Methods in Molecular Biology

Andrea Keszthelyi, Izumi Miyabe, Katie Ptasińska, Yasukazu Daigaku, Karel Naiman, Antony M. Carr (Role: Joint author, p239-259)

Humana Press, New York, NY, Jan, 2018 (ISBN: 9781493973057)

Presentations

Global usage of replicative polymerases and its relation with transcription in human cells

UK-Japan Genome Stability Symposium, Jan 20, 2023 Invited
The balance of flexibility and fragility in DNA polymerase usage and replication fork dynamics

Dec 1, 2022 Invited
Genome-wide profiling of DNA polymerase usage and replication kinetics in human cells

NIG International Symposium 2022 Chromosome Replication in the New Era, Nov 12, 2022 Invited
DNA polymerase dynamics and genomic instability

大学保一, 鐘巻将人

第81回日本癌学会学術総会, Sep 29, 2022 Invited
遺伝子分布・転写機構が複製フォーク動態に及ぼす影響

大学保一, 小柳恵理, 柿本洋子, 南澤宝美后, 夏目豊彰, 鐘巻将人

日本遺伝学会第94回大会, Sep 14, 2022
DNAポリメラーゼ動態から見る，転写機構の染色体複製への影響

小柳恵理, 柿本洋子, 荻朋男, 夏目豊彰, 鐘巻将人, 大学保一

第39回染色体ワークショップ・第20回核ダイナミクス研究会, Dec 21, 2021
遺伝子転写のDNAポリメラーゼ動態への影響

小柳恵理, 柿本洋子, 荻朋男, 夏目豊彰, 鐘巻将人, 大学保一

第２６回DNA複製・組換え・修復ワークショップ, Oct 22, 2021
The diversity of replication fork dynamics in the nuclear environment

Sep 8, 2021 Invited
Profiling DNA polymerase functions and exploring chromosome structures

DAIGAKU Yasukazu

Dec 22, 2019
ヒト細胞のおけるＤＮＡ複製動態の柔軟さ，危うさ

大学保一

第４２回分子生物学会年会, Dec 3, 2019 Invited
DNAポリメラーゼ機能から見るヒト細胞でのゲノム複製機構

大学保一

第２５回DNA複製・組換え・修復ワークショップ, Nov 9, 2019
Identification of replication initiation sites in human cells using the genome-wide DNA polymerase profile

DAIGAKU Yasukazu

Sep 11, 2019
Global usage of replicative DNA polymerase in human cells

Yasukazu Daigaku

Cold Spring Harbor Laboratory Meeting "Eukaryotic DNA Replication & Genome Maintenance", Sep 6, 2019
染色体構造とＤＮＡ複製動態 -分裂酵母とヒト細胞の視点から-

大学保一

染色体研究の最前線2019, Mar 8, 2019
複製フォーク動態のゲノム科学的解析から見る染色体複製

大学保一

第３６回染色体ワークショップ, Jan 25, 2019
Flexibility in DNA polymerase functions during genome duplication

DAIGAKU Yasukazu

Nov 30, 2018 Invited
Usage of error prone DNA polymerase during genome replication

DAIGAKU Yasukazu

11th 3R+3C Symposium, Oct 14, 2018
DNA 複製とゲノム安定性制御の研究

大学保一

日本遺伝学会第９０回大会（奨励賞受賞講演）, Sep 20, 2018 Invited
Insight into stochastic initiation of DNA replication through analysis of fork structures in S. pombe

DAIGAKU Yasukazu

Sep 20, 2018 Invited
DNA polymerase usage and mutagenesis

Yasukazu Daigaku

JSPS-DST Japan-India Forum for Advanced Study: Epigenetics and Human Disease, Feb 6, 2018 Invited

Teaching Experience

Oct, 2020 - Mar, 2021

Biology A (Tohoku University)
Jun, 2019 - Sep, 2019

基礎ゼミ「Interdisciplinary Sciences 〜分野を飛び越えて大学での学びを広げよう〜」 (東北大学)

Professional Memberships

Research Projects

RNA転写のDNA複製へのトランスアクション

科学研究費助成事業, 挑戦的研究(萌芽), 日本学術振興会, Jul, 2021 - Mar, 2023

大学保一
DNAポリメラーゼ動態から成る遺伝情報の安定性

科学研究費助成事業, 基盤研究(B), 日本学術振興会, Apr, 2020 - Mar, 2023

大学保一
Genome-wide analysis of cooperative action among DNA polymerases

Grants-in-Aid for Scientific Research, Grant-in-Aid for Young Scientists (A), Japan Society for the Promotion of Science, Apr, 2016 - Mar, 2020

Daigaku Yasukazu
Genome-wide identification of replicative DNA polymerase usage

Grants-in-Aid for Scientific Research, Grant-in-Aid for Challenging Research (Exploratory), Japan Society for the Promotion of Science, Jun, 2017 - Mar, 2019

Daigaku Yasukazu, Kanemaki Masato
DNAポリメラーゼ研究におけるゲノム科学的，分子科学的アプローチの融合

科学研究費助成事業, 研究活動スタート支援, 日本学術振興会, Aug, 2015 - Mar, 2016

大学保一

Profile Information

Research Interests

Research Areas

Research History

Committee Memberships

Awards

Papers

Misc.

Books and Other Publications

Presentations

Teaching Experience

Professional Memberships

Research Projects

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