論文

査読有り
2017年6月

Polyphyllin D, a steroidal saponin in Paris polyphylla, induces apoptosis and necroptosis cell death of neuroblastoma cells

PEDIATRIC SURGERY INTERNATIONAL
  • Shunsuke Watanabe
  • ,
  • Tatuya Suzuki
  • ,
  • Fujio Hara
  • ,
  • Toshihiro Yasui
  • ,
  • Naoko Uga
  • ,
  • Atuki Naoe

33
6
開始ページ
713
終了ページ
719
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s00383-017-4069-4
出版者・発行元
SPRINGER

Purpose Neuroblastoma is a refractory pediatric malignant solid tumor. The previous studies demonstrated that Polyphyllin D, the main constituent of Paris polyphylla, a traditional Chinese medicine, exerts an anti-tumor effect on many tumors. However, its effects against neuroblastomas are unclear.
Methods We examined the anti-tumor effect of polyphyllin D in human neuroblastoma using IMR-32 and LA-N-2 cells, which exhibit MYCN gene amplification, and NB-69 cells, which do not exhibit MYCN gene amplification.
Results All cell lines showed reduced cell viability in response to polyphyllin D treatment. No caspase-3/-7, -8, and -9 activity was observed in IMR-32 and LA-N-2 cells treated with polyphyllin D. In contrast, activation of caspase-3/-7, and -8 activity was observed in NB-69 cells. When polyphyllin D and specific inhibitors of RIPK3 involved in necroptosis were added to IMR-32 and LA-N-2 cell lines, polyphyllin D-induced cell death was inhibited.
Conclusion Together, this indicates that the underlying mechanism of polyphyllin D-induced cell death in NB-69 cells is apoptosis, whereas the cell death of IMR-32 and LA-N-2 cells occurs by necroptosis. We continue research on this topic and look forward the discovery of a new therapeutic agent for neuroblastoma.

Web of Science ® 被引用回数 : 12

リンク情報
DOI
https://doi.org/10.1007/s00383-017-4069-4
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000401631000009&DestApp=WOS_CPL

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